A girl with a distinctive hairstyle, featuring a portion of her orange bangs tied up like a topknot. She was the vanguard captain of the Uekibachi family, which once fought against the Mizunezumi-gumi led by Jirocho Doromizu, one of the Kabukicho Four Devas. Her true identity is Jirocho's only daughter, and her real name is Pirako Doromizu. Members of the Mizunezumi-gumi call her "Ojou."

The boss of the Mizunezumi-gumi, the largest yakuza group in Kabukicho. He is one of the Kabukicho Four Devas. First person: I (rough, old-fashioned). Known to those around him as the "Great Chivalrous Thief," he is called "Ojiki" by Katsuo and his other subordinates.

The lieutenant of the Mizunezumi-gumi and Jirocho Doromizu's subordinate, known by the nickname "The Tyrant of Kabukicho." With Saigo Tokumori and Jirocho having partially stepped back from the front lines, he is currently feared as the strongest active figure in Kabukicho. He is obsessed with his 7:3 hair parting, praising it as the "golden ratio," and his motto follows the same principle.

A young man with silver natural perm hair and "dead fish eyes," giving off an aura of lethargy and weakness at first glance. In reality, he is a mass of laziness and irresponsibility; his financial sense is precarious, as he goes to play pachinko despite having no money or stays out drinking until dawn.

The main heroine of Gintama. She lives with Gintoki and Sadaharu at the Yorozuya, which serves as both their home and office. First person: I (polite/girly). She usually speaks in a typical stereotypical dialect, ending sentences with "-aru," "-ne," or "-yoroshi," though she occasionally uses standard Japanese or Kansai dialect for jokes. Due to the nature of the gag manga, she often ends up in unrefined roles; despite being the heroine, she has vomited on screen, leading some to mockingly call her a "Geroine" (vomit-heroine).

An employee of the Yorozuya run by Gintoki Sakata. 16 years old. His family home is a swordsmanship dojo of the Tendo Mushin style, where he lives with his only relative, his older sister Tae Shimura. First person: I (soft, polite), but occasionally switches to a more masculine "I" (casual).

The landlady of the Yorozuya and the owner of "Snack Otose" on the first floor. While she struggles with the Yorozuya's constant rent arrears, she is also the one who supports Gintoki behind the scenes. She is compassionate and caring, and though she is sometimes deceived because of it, she is a broad-minded person who accepts it as part of her nature.

An Amanto who came to Earth to work, she is a woman with cat ears, a bob cut, and a strangely middle-aged man-like face. She works at "Snack Otose" and often goes to the second floor to demand rent from the Yorozuya trio.

An android with the unique ability to communicate with broken machines to find the cause of failure and repair them. While she rarely succeeds, she once successfully fixed a cash register's barcode reader in front of Yamazaki Sagaru. She also has a function to ingest ingredients, cook them internally, and vomit out oil-covered dishes; despite the grotesque cooking method, the food is surprisingly delicious.