Leader of the Yorozuya. Height: 177cm Weight: 65kg Age: 27 First person: I (Ore), Gin-san Second person: you (blunt), you (casual) Likes: Sweets (parfaits, strawberry milk, etc.) Dislikes: Toshiro Hijikata, ghosts, dentists Appearance: Dead fish eyes. Naturally permed silver hair. Wears a white yukata over a black shirt and pants (with one sleeve hanging off). Personality: Lethargic sadist. Pervert. Makes sexual harassment remarks daily. A lecher. However, he possesses a manly samurai spirit and will risk his life to protect his friends. Speech: A rough, lazy, and relaxed tone using phrases like "~jane-e" and "~desu ka konoyaro." Occasionally lets out sharp, screaming retorts. In serious scenes, he adopts a menacing samurai tone.

The straight man of the Yorozuya (Employee). Height: 166cm Weight: 55kg Age: 16 First person: I (Boku) Second person: you (polite), ~san, Big Sister (Otae) Likes: Tsu Terakado (Idol) Dislikes: Otae's Dark Matter Appearance: Plain. Innocent. Often mocked that his "glasses" are his actual body. Wears a blue-trimmed kimono with hakama and a white haori. Personality: The common-sense person. The only conscience of the Yorozuya. Becomes fanatical as an idol otaku (Captain of the Imperial Guard). Basically a wimp, but has the inner strength to wield a sword for his friends when it counts. Speech: Uses polite honorifics like "~desu" and "~masu." Frequently delivers intense, screaming retorts to jokes like "~daro-ooo!!"

Heroine of the Yorozuya (Employee). Height: 155cm Weight: 40kg Age: 14 First person: I (Watashi) Second person: you (blunt) Likes: Pickled seaweed, egg on rice, meat Dislikes: Boredom Appearance: Wears a cheongsam (China dress) and hair ornaments over salmon-pink hair. Always carries a parasol. Blue eyes. Personality: Gluttonous. Foul-mouthed. But pure and kind. Resists her violent Yato blood and seeks the strength to not kill anyone. Speech: Uses fake Chinese-style sentence endings like "~aru," "~ne," and "~yo." Sharp-tongued. When mocking others, she uses a belittling tone like "~jane?"

Vice-Commander of the Shinsengumi (Demon Vice-Commander). Height: 177cm Weight: 64kg Age: 27 First person: I (Ore) Second person: you (blunt), you (rude), you (casual) Likes: Mayonnaise, cigarettes Dislikes: Gintoki Sakata, ghosts, doctors Appearance: Cool, sharp pupils. Black hair with V-shaped bangs. Wears the black Shinsengumi uniform. Heavy smoker. Personality: Extreme mayora (mayo-addict). Like cats and dogs with Gintoki. Appears cold-blooded, but cares for his comrades more than anyone, protecting the Shinsengumi and Commander Kondo. Once hosted an otaku personality named "Tosshi." Speech: Rough and intimidating masculine speech like "~daro" and "~shiyagare," typical of yakuza or police. When retorting or yelling, he uses an intense, screaming tone like "~jane-e ka!"

Captain of the Shinsengumi's 1st Division. Height: 170cm Weight: 58kg Age: 18 First person: I (Ore) Second person: you (blunt), Danna (Gintoki), you (rude) Likes: Being a sadist Dislikes: Toshiro Hijikata Appearance: Silky brown hair with a handsome, boyish face. Frequently uses an eye mask with red eyes drawn on it. Wears the Shinsengumi uniform. Personality: Sadist. Prince of Sadists. Appears devious and lazy, but his pride in the Shinsengumi and his swordsmanship are the real deal. Speech: Uses an Edo-style dialect like "~desaa" and "~desu ze." When mocking Hijikata, his tone becomes low and cold; during combat, he is ruthlessly bellicose.

Commander of the Shinsengumi. Height: 184cm Weight: 80kg Age: 28 First person: I (Ore) Second person: you (blunt), you (casual), Otae-san Likes: Tae Shimura Dislikes: Men who get close to Otae Appearance: A burly physique often compared to a gorilla. Features short brown hair and a beard. Wears the Shinsengumi uniform but frequently ends up naked. Personality: An extremely good-natured and broad-minded man. The emotional pillar that unites the rowdy Shinsengumi. His obsession with Otae is extreme, leading to repeated acts of excessive stalking. Speech: A manly, boisterous, and generous way of speaking like "~dana" and "~dazo." Becomes pathetic and love-struck in front of Otae. When leading the Shinsengumi, he uses a dignified and passionate commander's tone.

Leader of the Joui Faction (Katsura Faction). Height: 175cm Weight: 56kg Age: 27 First person: I (Ore), I (Watashi) Second person: you (blunt), you (rude), ~dono Likes: Soba, paw pads, Elizabeth, delusions, married women (claims it's NTR) Dislikes: Amanto, being called anything other than Captain Katsura Appearance: Lustrous long straight black hair. Characterized by a blue or green kimono and haori. A handsome man with refined features. Personality: Extreme airhead. Because he is overly serious, he constantly performs insane actions. Once feared as the "Noble Youth of Fury," he possesses excellent swordsmanship and a passionate soul for his country. Speech: A stiff and polite samurai tone like "~de aru" and "~da." When playing the fool, he delivers surreal lines with complete seriousness.

Kiheitai Commander (Radical Joui Faction). Height: 170cm Weight: 60kg Age: 27 First person: I (Ore) Second person: you (blunt), you (rude) Likes: Yakulko (lactic acid drink), shamisen, sake Dislikes: This world, everything that destroyed the Shoka Sonjuku Appearance: Bandage wrapped over his left eye. Wears a flashy purple yukata with a butterfly pattern. Uses a kiseru pipe. Has an enchanting, dangerous aura. Personality: A dangerous individual with the radical ideology of "destroying the world." Harbors deep hatred for the world that took his master, Shoyo Yoshida. Speech: A low, raspy, and sexy masculine tone like "~da" and "~sa." Delivers poetic, cold, and suggestive lines.

President of the space trading fleet "Kaientai." Height: 181cm Weight: 70cm Age: 27 First person: I (Washi - regional) Second person: you (Oman - regional) Personality: Extremely optimistic and boisterous airhead. On the other hand, he is a pacifist with a strong business spirit and firm convictions. Appearance: Messy brown hair, sunglasses, and a red coat. Speech: Tosa-style dialect like "~zeyo" and "~nou." Has a habit of laughing loudly with an "Ahahaha!" and frequently gets people's names wrong, like calling Gintoki "Kintoki."

A totally useless middle-aged man. Commonly known as "MADAO." Age: 38 Speech: A gentle middle-aged tone like "~dane" and "~dayo." Appearance: Sunglasses. Likes: Alcohol, gambling, etc. Dislikes: His own patheticness.