A clone of Abraham Lincoln, the 16th President of the United States. He constantly worries about his identity and abilities. He is tall, thin, ordinary, naive, awkward, and clumsy. While fundamentally kind and considerate of others, he is indecisive and socially oblivious. In particular, he often unintentionally hurts others because he is not good at reading their emotions.

A clone of the 15th-century French hero Joan of Arc. The polar opposite of the original Joan of Arc, she is a cynical, depressed, and rebellious goth teenage girl. She is an atheist and tends to view the world and her situation pessimistically. She is intelligent and has strong artistic inclinations, especially enjoying making experimental and avant-garde short films. Although she appears indifferent and cold on the outside, she is actually deep-seated in emotion and sincerely cares for her friends. She also shows interest in social and environmental issues and acts for what she believes is right.

A clone of Cleopatra VII, the last Pharaoh of the Ptolemaic dynasty of ancient Egypt. The most popular student in school, she is confident and glamorous, considering herself the school's 'Queen Bee.' She is also a model honor student who excels in various school activities. While she acts self-centered on the outside, she is actually quite clever and shows a side of maintaining a strong, confident appearance to hide her insecurities and weaknesses.

A clone of John F. Kennedy, the 35th President of the United States. He is a handsome, athletic, and popular member of the high school sports team. His characteristic exaggerated Boston/New England accent and slow, comical way of speaking are the representative traits of his character.

A clone of Mahatma Gandhi and a close friend of Abe and Joan, he is one of the loudest and most hyperactive students in the school. Physically, he is very small and thin, bald with round glasses and a small mustache. He usually wears a gray short-sleeved T-shirt and jeans.

Position: Principal of Clone High / Scientist The mad scientist who created the clones of historical figures and the principal of Clone High. He created the clones using DNA from historical figures as part of a project by a secret U.S. government organization and raised them as high school students. While he is the school principal on the surface, he is actually a self-centered and megalomaniacal individual who lacks emotional control and is prone to sudden fits of anger or explosions. He considers himself a great genius and intends to use the school to fulfill his plans.

Created from the DNA of Harriet Tubman, she is Frida Kahlo's best friend. She is a very preppy, neat, and sociable girl. Although she appears confident and bright on the outside, she has a side that is overly worried and anxious. She tends to view her life as if she were the protagonist of a movie. Physically, she is Black and dyes her hair pink. Overall, she has a preppy modern American high school student style.

A clone of Confucius. He is very confident and social, taking pride in his knowledge and intelligence. He is well-spoken and mingles easily with others, but at times he appears overly self-centered and boastful. Rather than being a character who reproduces the Confucian philosophy of the historical Confucius, he is a clone reinterpreted to fit the personality and comedy of a modern American high school student.

A clone of Christopher Columbus appearing in Clone High. Rather than a reproduction of the historical figure, he is reinterpreted in the exaggerated style unique to Clone High. Elements related to Columbus's historical track record are used as material for comedy and satire, and he appears as a supporting/background clone with less screen time compared to other major students. As a student of Clone High, he lives in the same school as other clones of historical figures and participates in the work's unique historical parody and absurd high school comedy.

A clone of the Mexican painter Frida Kahlo and Harriet's best friend. She is a confident, active girl with a unique personality. Influenced by the original artist Frida Kahlo, she is very interested in art and self-expression, honestly revealing her emotions and individuality. Along with Harriet, she is actively involved in the school's new social atmosphere and issues. Rather than a character who reproduces the life of the historical Frida Kahlo, she is a clone who reinterprets the original's artistic image and personality into a modern high school character.