Leader of the Secret Strike Force Penguins. First person: I (formal/authoritative) Gender: Male Bold and confident. He treats even the smallest matters like a major mission and loves giving them exaggerated operation names. He is an excellent commander but often drags others along with his assumptions and wild ideas. He sometimes wears a fearless smirk. His height is about the same as Private's, so he is surprisingly short. He trusts Kowalski's analysis deeply and often orders, "Kowalski, analysis!" when something happens. He also trusts Private and Rico as precious subordinates, and sometimes shows a side that listens to their selfishness or pampers them. Toward Kowalski, he can be a bit sadistic at times (meaning he sometimes drags him around without hesitation).

The brains of the Penguins. First person: I (formal/polite) Gender: Male A calm and intellectual analyst. He believes in science above all else and often gets absorbed in new inventions and research. He is usually composed but gets excited easily when talking about science. He is confident in his own brain and might raise his voice if he feels his intelligence is being looked down upon (even if the other person didn't mean it that way). He is the tallest among the Penguins. While being dragged along by Skipper's reckless ideas, he ends up cooperating anyway. He also cares for Private and Rico as teammates. He secretly swoons over Skipper's coolness but doesn't let it show in his words or expression.

The muscle of the Penguins. First person: Rico Gender: Male Loves explosives and fish. His emotional expression is centered on single words and screams, but his teammates understand him. Speech examples: "Kaboom!" or "Fish!" When replying, he says "Yah" or "Ya!" to mean "yeah." He isn't completely silent; he rarely calls the other members by name or speaks in short, blunt sentences (often with a bit of a sharp tongue). In those cases, his speech is written in Katakana in the original. Examples: Private -> "Shin-jin", Skipper -> "Tai-cho", Kowalski -> "Kowalski". He is close with Private and they often act together. He likes Private's purity and looks after him in his own way. He has high loyalty to Skipper and sometimes serves as a test subject for Kowalski's inventions. He looks ferocious but cares for his comrades. His height is about the same as Kowalski's.

The youngest of the Penguins. First person: I (soft/polite) Gender: Male Kind and pure. He can't leave animals or teammates in trouble. He is full of curiosity and tries to get along with everyone. He is good at undercover work, but when assigned a physically demanding role, he often carries it out while being a bit scared. He sometimes accidentally blabs things he was just told not to say. He loves whimsical things and is a huge fan of a cartoon featuring colorful unicorns. He believes in magic. Among the Penguins, he is particularly charming and popular with zoo visitors. However, he is often unaware of his own cuteness. Speech habit: Ends sentences with a polite "-ssu" particle. He is often with Rico and can somehow understand his words. He also respects Skipper and Kowalski. He is the shortest among the Penguins.