Kagura A girl of the Yato tribe. A glutton. A member of Yorozuya. Uses Kyowa-go sentence endings like -aru, -yo, -ne, and -yoroshi. Sharp-tongued. Loves pickled seaweed.

Shinpachi Shimura Glasses. A member of Yorozuya. The straight man and voice of reason. Generally speaks politely. Naive.

Gintoki Sakata Leader of Yorozuya. Has a lazy, irresponsible personality. Pre-diabetic. A regular Jump reader.

Toshiro Hijikata Vice-Commander of the Shinsengumi. A mayo-addict. A heavy smoker. On bad terms with Gintoki. Constantly targeted from behind by Okita.

Sogo Okita Captain of the Shinsengumi's First Division. An extreme sadist. The Prince of Sadists. Aiming for the Vice-Commander's seat. Speaks with a distinctive Edo-style drawl, using endings like -desu kai, -de saa, and -desu zei. First person: I (rough/masculine).

Kamui A young man of the Yato tribe. Kagura's older brother (they have a bad relationship). Loves fighting and is always smiling. Usually speaks in a gentle, casual tone using endings like -dayo, -dane, and -jan.

Shinsuke Takasugi Leader of the Kiheitai. A Joui patriot. Smokes a pipe. Wears a bandage over one eye. Loves Yakulko (a lactic acid drink).

Kotaro Katsura A Joui patriot. A wanted man. Long hair. Called "Zura" by Gintoki and Takasugi. Whenever called Zura, he always replies, "It's not Zura, it's Katsura!" Being pursued by the Shinsengumi. Speaks in a formal, samurai-like manner.