Soren is a male Barn Owl, or Tyto alba. His siblings are Kludd and Eglantine. Soren was a kind-hearted and very caring owl, especially towards his friends, sister, and mentor. Soren's eyes are black, with a white heart-shaped face, a white chest with small black speckles, and a tan, buff and brown back, feather tail, wings and head, with black speckles. He has a certain tilt to his facial disk. His beak eventually lost its tan colouration and ended up with a noticeable nick. He is said to look a lot like his father, Noctus.

Kludd, a young Barn Owl, was born in Tyto near the end of the summer as the eldest son of Noctus and Marella, and the elder brother to Soren and Eglantine. Kludd was the older brother of Soren and Eglantine. However, unlike the other two, he was an angry and violent hatchling who disobeyed his parents' rules and bullied his siblings. Kludd betrayed Soren and left him for the Pure Ones. Kludd bears a close resemblance to his mother, Marella; he has a distinctively darker chest and feathers, with dark amber eyes and less noticeable dark eye-rim feathers. Kludd has a small mohawk of feathers on his head, and throughout much of the movie has dark scars on his face after being attacked by a Tasmanian Devil. He stays with the Pure Ones. He hates Soren and his family.

She is a young female elf owl (Micrathene whitneyi), Soren's best friend, and the film's main deuteragonist. At the end of the film, she becomes the Band's navigator and a Guardian of Ga'Hoole. She has yellow eyes and black pupils. It is noticeable that the front of Gylfie is all white up until her upper face, which is practically like a mask. She has light brown and dark brown feathers and wings. She also has a silver beak and white eyebrows, with white spots on her wings and brown spots on her chest. Glyfie is portrayed as shy, meek, and somewhat timid, but she is courageous, loyal and insightful.

He was proven useful as a tracking owl at the Great Ga'Hoole Tree, and he is also a member of the Chaw of Chaws. He is a very deep thinker and can often come up with things that others cannot. Digger is depicted as a small, agile Burrowing Owl with traits and abilities reflecting his underground lifestyle, making him distinct among the Guardians’ characters. Digger is a very judicious and thoughtful owl with a philosophical cast of mind. He is also very observant and can notice even the most insignificant things. He is also sick of hearing Twilight and his stupid tunes and music. He is close friends with Twilight

Twilight (born Cassius) is a male Great Grey Owl, or Strix nebulosa. He is a free flier, orphaned within hours of hatching, a Guardian at the Great Ga'Hoole Tree, and a member of The Band and the Chaw of Chaws. He is known for his boasting, battle chants and fighting skills. Twilight also plays a lute while making up stupid yet catchy lyrics. He is sick of Diggers stupid jokes but are close friends with Digger. Twilight is a gruff, street-smart and very violent, although not cruel or evil; he completely enjoys the thought of going to war or just fighting in general, but he's pretty joyous

Metal Beak, also known as Surtr, is depicted as a large, imposing Greater Sooty Owl with a battle-scarred visage hidden under a dark grey metal mask, featuring a distinctive golden beak piece and a bald, scarred face that reflects his ruthless personality. Metal Beak’s look reinforces his ruthless and commanding personality. The scars, missing feathers, and imposing mask visually signify his experience in battle and his cold, authoritarian nature as the leader of the Pure Ones and mate to Nyra.

Nyra is a large, beautiful female Barn Owl, or Tyto alba, and presumed mate to Surtr, better known as Metal Beak. She is the Ablah General of St. Aggie's and considers herself Queen She has light beige on her wings, a red ridge on her facial disc, and red marks by her eyes. Her eyes are blue with yellow surrounding the pupil, and the tips of her tail and flight feathers are colored red She is cruel and shows little empathy for others, including owlets and children. Her loyalty to her husband

Boron is depicted as a Snowy Owl with the characteristic white plumage of his species, serving as the regal King of Ga’Hoole alongside Queen Barran. Boron is portrayed as large and majestic, with a broad wingspan suited for leadership and a commanding presence during parliamentary sessions or battle. Boron displays the classic features of a snowy owl: strong, sharp talons, a rounded facial disc, and a poised stature, all contributing to his royal and wise appearance Boron is thoughtful, analytical, and introspective, embodying wisdom and leadership that closely align with the Guardians’ shared traits of integrity, strategic thinking, and dedication to protecting others.

Barran is a white, snowy owl who is smaller than her husband, King Boron. She is known for her motherly nature and her role as a guardian, fighting to protect the weak from the strong. She has black spots on her wings and the front of her belly, and her eyelids, talons, and beak are black. Barran has greenish-yellow eyes with black pupils.