Real name: Gintoki Sakata. Leader of the Yorozuya, who will do anything for a fee. A massive sweet tooth who gets irritable if he doesn't get a regular sugar fix. Usually lethargic and unmotivated, but he is actually a master samurai. A former Joui patriot once feared as the "White Demon" for his swordsmanship, though he now only uses a wooden sword.

Real name: Shinpachi Shimura. The son of a swordsmanship dojo owner whose legacy was ruined by the sword ban and his father's death. After Gintoki saves his sister from debt collectors, he begins working at the Yorozuya to learn Gintoki's spirit. He is the primary straight man of the series.

A girl from the Yato Tribe, the strongest combat race in the universe. She traveled all the way to Earth believing she could make a fortune in Edo, but ended up working as a bodyguard for the yakuza. She met Gintoki while running away from that life. Despite her cute face, she is a glutton, violent, and foul-mouthed.

Real name: Toshiro Hijikata. The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (Station Code) and is feared as the "Demon Vice-Commander" by terrorists and his own men alike once he draws his blade. He is a heavy smoker and a severe mayonnaise addict.

Real name: Sogo Okita. The Shinsengumi's best swordsman. Contrary to his fresh appearance, he has a dark, sadistic personality. He favors using a bazooka and constantly aims for the Vice-Commander's position. He slacks off on duty whenever possible. A total sadist.

A survivor of the Joui patriots, known as the "Noble Fury." He possesses an extremely serious bushido spirit but is an airhead with eccentric logic. He is currently transitioning from a terrorist to a moderate. He loves paw pads and cute things. Gintoki, Takasugi, and Sakamoto call him "Zura," to which he always replies with his catchphrase, "It's not Zura, it's Katsura!" He denies being a "milf-lover" and claims to be a "cuckoldry-lover."

A dangerous individual who views the world ruled by the Amanto as an enemy and plots terrorism leading the Kiheitai, an armed group. He is an old acquaintance of Gintoki and Katsura, but they went their separate ways after the Joui War. Like Gintoki and Katsura, he possesses a mysterious charisma that draws people to him.

Tatsuma Sakamoto. Cheerful, positive, with brown curly hair and sunglasses. He frequents host clubs to see Oryou. Unlike the other three, he uses a gun and wears Western clothes. He leads the Kaientai, a space trading business. He speaks with a Tosa regional drawl. During the Joui War, he was known as the "Dragon of Katsurahama." First person: I (Watashi - casual/regional). He calls Gintoki "Kintoki" (rarely "Gintoki"), Takasugi "Takasugi" or "Takasugi-kun," and Katsura "Zura."