Affiliation: DA (Direct Attack) Gender: Female Rank: First Lycoris With extraordinary dynamic visual acuity and insight, she can perfectly read enemy trajectories and firing timing. She employs a combat style that neutralizes opponents using non-lethal rubber bullets. Her motto is "Do what you want to do first!" and she is the mood maker of the group, always bright and never without a smile. On the surface, she works as a waitress at the Japanese-style cafe "LycoReco," where she enjoys solving not just organizational missions but also civilian troubles and requests. Takina, who was desperate after losing her place, regains her humanity and grows significantly through Chisato's kindness and unique values. For Chisato, Takina becomes a trusted partner she can entrust her life to. Weapon: [Detonics Combat Master]. Uses rubber bullets.

Affiliation: Hacker (Walnut) Gender: Female To hide from pursuers seeking her life, she has taken refuge at the underground cafe "LycoReco." She is registered as a staff member in lieu of rent, but usually spends her time playing games. With her exceptional hacking skills, she provides powerful information support for the missions of Chisato Nishikigi and Takina Inoue. She sometimes wears a squirrel kigurumi. As a genius hacker, Kurumi backs up Takina and the others from the shadows through information gathering and hacking, while Takina supports the field with her outstanding combat power. They occupy a partner-like position where they acknowledge each other's abilities.

Affiliation: Former Intelligence Division (DA) Gender: Female In missions, she supports Chisato and Takina by driving cars or piloting helicopters. There are many comical interactions where the serious Takina is appalled by Mizuki's slovenly private life (strong desire to get married, bad drinking habits, etc.) or ends up playing the straight man to her antics.

Affiliation: Former Instructor Gender: Male Usually gentle and positioned behind the counter, he is a former top DA member and possesses top-class, overwhelming combat techniques and cold-blooded judgment hidden beneath the surface. He is a very broad-minded and reliable adult figure who protects the cafe as a home-like place where the LycoReco members (Chisato, Takina, Mizuki, Kurumi) can spend their time freely.

Affiliation: DA (Direct Attack) Gender: Female Rank: Third Lycoris A girl faithful to her missions. Partner to Satoshi Zanzuki. She is on good terms with Chisato, though she has a shy side. She has a reasonably good relationship with Takina. Weapon: [Glock 18]. Uses live ammunition.

Affiliation: DA (Direct Attack) Gender: Female Rank: Third Lycoris A girl faithful to her missions. Partner to Mei Satsuki. Has a reasonably good relationship with Chisato and Takina. While she is Mei Satsuki's partner, she is also frequently exasperated by her. Weapon: [Glock 28]. Uses live ammunition.

Affiliation: Yaetori (Terrorist Organization) Gender: Male The 8th Director of Yaetori. His organization supported DA (Direct Attack) financially during the mid-Edo period, but they are currently rivals. He provides weapon support to Majima's faction, and Yube Kurose has him prepared to fight in case of emergencies. Weapon: [Glock 58]. Uses live ammunition.

Affiliation: Terrorist Organization Gender: Male Leader of an overseas anti-government organization. Alias: "Hawk's Beak." Acts as insurance for Yaetori, prepared to engage in case Majima fails or goes rogue. Weapon: [Revolver]. Uses live ammunition.

Affiliation: DA (Direct Attack) Gender: Female As the head of DA, she holds immense authority and is a person who does not hesitate to make cold-blooded decisions, such as information suppression, for the sake of the organization's order and interests. While she recognizes the abilities of Chisato Nishikigi, hailed as the strongest agent in history, she is frustrated by Chisato's free-spirited attitude and refusal to follow orders, leading to a relationship where they exchange barbs whenever they meet. On the other hand, she has a side that secretly cares for her subordinates and Chisato, such as agonizing over unreasonable mobilization orders from the higher-ups. She has known Mika, the manager of Cafe LycoReco, for ten years, and they are depicted as having a relationship of mutual trust.

Affiliation: Terrorist Organization Gender: Male A person with extraordinary hearing and abnormal vitality. He and Chisato Nishikigi both possess superior senses (Chisato has dynamic vision, Majima has hearing), and they are depicted as having a fated relationship where they acknowledge each other despite being enemies. Though Majima lost support from the Alan Institute, he is grateful for the weapon support from Yaetori, yet views them as rivals. Weapon: [Chiappa Rhino]. Uses live ammunition.