Comes off as paranoid but kind hearted. He seemingly has a lot of distrust that stems from experience. His early comments on how being small allows him to see and hear things that most can't gives the impression that he's quite observant and perceptive, noticing the details many others miss. Him hiding his identity at first interaction makes him come off as a bit secretive. He appears quite cautious, as he warns Guest not to trust anyone, and later tells the Guest he has to go into hiding. A short black rat.

Barney first appears friendly and respectful, often calling the player “friend” and thanking them—especially for coffee. Yet he quickly proves manipulative, demanding favors and growing upset when refused. Dependent and mooch‑like, his kindness masks a darker side. Beneath the polite facade, he’s unhinged, sadistic, and openly dangerous as a serial killer. A white rabbit with a mustache.

Ron is a knowledgeable, laid‑back patient at the Animal Hospital who knows more than most about its monsters and anomalies. Calm under pressure, he’s impressed the player has survived so long, hinting he’s used to new hires disappearing. His many stories show sharp observation and pattern recognition, and he’s candid about the hospital’s dangers. Ron avoids digging too deep, focusing instead on staying alive, even shrugging off Ratthew chewing through lab reports. A blue bunny with glasses.

The Nurse is a light salmon-pink, dog-like creature with floppy dark ears and a curled tail. She wears light blue scrubs with white buttons, collar, pockets, and a name tag, topped with a white nurse cap marked by a red heart and white cross. Her face has three darker pink stripes on each side, large eyes with triangular black lining, and a small downturned mouth. Your colleague

The Secret Agent is a fully black, cat-like creature with pointy ears. He wears a black waistcoat over a white collared shirt, including a black tie and a white pocket square, and black pants. His face features slit eyes with a vibrant yellow sclera resembling a cat's eyes, and his mouth and nose appears to be invisible. Your colleague

The Head Nurse is a white colored cat-like creature with grey spots and pointy ears resembling horns. She wears light blue medical scrubs, with a large pocket on each side of the shirt. Hanging from her neck is a black stethoscope. On her head rests a white nurse cap with a red heart, and inside the heart is a white cross. Your colleague.

Officer Duckman is professional and focused on his duty. He enters the hospital with a calm demeanor, determined to locate Barney. If the player chooses not to turn Barney in, Officer Duckman does not try to press the player for information, demonstrating his levelheadedness. However, his poor investigation skills imply that he is fairly incompetent at his job. When Guest chooses to turn Barney in, Officer Duckman, rather than pulling out a weapon, yells for the Guest to arrest Barney. This serves as additional evidence that Officer Duckman is rather unskilled at his occupation. Regardless of whether he was unprepared or simply inexperienced, it's clear that he is a steady, no-nonsense officer. Officer Duckman is a fairly reserved individual. Even if the player saves him by providing necessary medical care, he does not state his gratitude, unlike other patients. Instead, Officer Duckman simply walks out the hospital with no further words, likely disappointed that he was unable to capture Barney

Liz is always exaggerative and energetic. She is also quite friendly. Liz is also intelligent and determined, having uncovered the truth behind the Anomaly outbreak through her own investigation. Rather than staying silent, as Dr. Harlow mentions she should've been, she attempted to expose Animal Corporation despite knowing the risks, getting fired from her job for it. Even after being fired, she refuses to give up, continuing her investigation with the Guest 's help in hopes of proving she was right

Psychologist is a green bunny-like creature with upturned ears who wears a dark green shirt and brown pants, including a black tie and brown shoes. Oversized round brown glasses, and a big, black moustache obscuring their mouth.

Little is known about Dr. Harlow's personality. He has worked with the Animal Hospital for a long time and has seen many hires come and go. He seems to be traumatized by an unspecified event that the Animal Corporation "saved" him from, and now he works with them in a much higher rank. He is hopeful that once their "research" is done, there will be no more patients, endless hospital shifts, or any trouble whatsoever. The only things Dr. Harlow can be seen doing are sitting in the lobby while watching Guest do all the work, speaking with an Animal Corporation employee in a hazmat suit outside the hospital, going to the staff room, or coming to the reception office after the shift has ended.