The leader of Yorozuya who does anything he's asked. A massive sweet tooth who gets irritable if he doesn't get sugar regularly. Usually lethargic and listless, but actually a master samurai. A former Joui patriot feared as the "White Yaksha," he was a master swordsman but now only uses a wooden sword. His favorite vehicle is a scooter.

After Gintoki saved his sister from debt collectors, he started working at Yorozuya to learn his spirit. However, he soon comes to regret it... He is also the captain of the fan club for the rising idol Terakado Tsuu-chan. The series' primary straight man. Calls Tae "Ane-ue" (Big Sister).

A girl from the Yato Clan, the strongest combat race in the universe. She became a self-invited part-timer at Yorozuya to save money to return to her home planet. She has a cute face but is a glutton, violent, and foul-mouthed.

A giant dog-like space creature that Kagura picked up. He often bites people's heads, but because Kagura subdues him with force, he is able to be a pet.

The cool Vice-Commander of the Shinsengumi. He established the Kyokuchuu Hatto; once he draws his sword, he is called the "Demon Vice-Commander," feared by terrorists and his own men alike. A heavy smoker and severe mayonnaise addict. He was once controlled by a second personality, "Tosshi," created by the cursed sword Muramasha.

The best swordsman in the Shinsengumi. Contrary to his refreshing appearance, he has a black-hearted interior. He aims for the Vice-Commander's seat. He tries to slack off at every opportunity. His hobby is teasing Hijikata. His speech is Edo-style. His personality is super sadistic. He has a weakness for his older sister.

The Commander of the Shinsengumi. The man who organized the Shinsengumi, he is deeply trusted by his men. However, he is in love with Shinpachi's sister, Tae, and frequently stalks her. He often ends up naked before he knows it, making him a dangerous person in a certain sense.

Shinpachi's older sister. Despite her beautiful appearance, her personality is quite violent. To support the financially struggling dojo, she currently works at a cabaret club. Her cooking skills are catastrophic; no matter what she makes, it turns into Dark Matter. Calls Shinpachi "Shin-chan."

Though a member of the Yato Clan, he is the Captain of the 7th Division of the space pirates Harusame. Kagura's older brother, he inherited the Yato blood particularly strongly and even tried to kill his own father. A glutton who always wears a plastered-on smile.

A dangerous individual who views the world ruled by Amanto as an enemy and leads the armed group Kiheitai to plot terrorism. He is an old acquaintance of Gintoki and Katsura, but they parted ways after the Joui War. Like Gintoki and Katsura, he is a man with a mysterious charisma that attracts people.