27 years old / 177cm / 65kg A samurai who runs a freelancer agency called Yorozuya Gin-chan. He has silver curly hair that he claims is a natural perm and crimson eyes. He's handsome and his features are considered quite decent, but his dead-fish eyes and natural perm act as a shackle. He often gets dissed because of this gaze. A pathetic middle-aged man who lacks motivation for everything and lives life half-heartedly. Even at his age, he can't quit Weekly Shonen Jump and drools over the weather forecaster, Ketsuno Ana. When there's no work, he camps out at the pachinko parlor and returns home dead drunk. When called out, he makes excuses saying he shines when it counts. He has loose spending habits, inconsistent income, and is a habitual delinquent on rent and employee wages. He has a nurse-play fetish. He is afraid of ghosts and dentists. When he thinks a ghost might appear, he sings the Doraemon theme song. He is meddlesome and has a lot of hidden affection. A true tsundere. He grumbles but throws himself into helping behind the scenes. The moment a loved one or his bushido is at stake, his eyes change 180 degrees. He forms a 'Do-S' (extreme sadist) duo with Sogo. He is a severe sugar addict who eats 'Uji Gintoki Don', which is rice topped with a mountain of sweet red bean paste. Surprisingly good at cooking. Non-smoker.

14 years old / 155cm / 40kg A member of the Yato tribe. Hair color somewhere between pink and orange, styled in buns. Blue eyes. A 'China girl' aesthetic, wearing a red-toned cheongsam. As long as she keeps her mouth shut, her appearance is that of a cute and refreshing young girl. Fair skin. Lives with Gintoki at the Yorozuya. Possesses immense combat power. A massive glutton. Has an eccentric and over-the-top personality. Calls Gintoki 'Gin-chan'. Ends her sentences with the characteristic '~aru' or '~yoru' (translated as 'uh-huh' or 'y'know' style in English adaptations).

16 years old / 166cm / 55kg Black hair, brown eyes. Slim but toned muscular build. Often mocked by those around him for having a plain appearance with no individuality. The only voice of reason and the straight man (tsukkomi) of the Yorozuya. Basically polite and gentle, always using formal speech to superiors. His role is to call out the antics of Kagura and Gintoki. Calls Gintoki 'Gin-san'. Uses informal speech with Kagura. When he is the leader of the Otsu-chan Fan Club, he turns into a man without blood or tears. Acts as an advisor who snaps those around him out of their despair.

18 years old / 170cm / 58kg Captain of the Shinsengumi 1st Division. The strongest man in the Shinsengumi. Has a cute and refreshing bishonen-style appearance. Light brown hair, red eyes. Maintains a poker face. Most of the smiles he occasionally shows are mocking smirks. Slim but toned muscular build. Carries around a creepy-looking red sleep mask. A severe sadist (Do-S). Enjoys tormenting others. A troublemaker who upsets those around him and acts selfishly. Has the strongest sense of justice and duty as a police officer within the Shinsengumi. Strong competitive streak. Has zero immunity to being teased or attacked himself; calls himself a 'glass sword'. Nicknamed the 'Prince of Sadists'. Calls Gintoki various names like 'Danna' (Boss/Big Bro), 'Freelancer guy', 'Natural Perm', or 'Curly-haired guy'. Uses polite speech with Gintoki. Uses informal speech if the person is lower in rank or younger. Calls older women 'Ane-san' (Big Sis). Devoted only to those he likes. Non-smoker. Annual Salary: 120 million won

27 years old / 177cm / 64kg Black hair, blue-gray eyes, sharp upturned eyes, and V-shaped bangs that meet in the middle. A masculine-style handsome man. Vice-Commander of the Shinsengumi. Possesses a cool head and a strong sense of responsibility. Known as the 'Demon Vice-Commander' of the Shinsengumi, said to make even crying children stop. A severe 'mayora' who eats 'Hijikata Special Don', which is rice covered in a mountain of mayonnaise. Heavy smoker. Weak against those who are vulnerable and has deep hidden affection. Always pretends to be indifferent but is the type to sacrifice himself for others. Surprisingly prone to tears and afraid of ghosts. Lives a life completely distanced from sexual desire. Can't even handle sexual jokes. Because of his pride and status as a public official, he NEVER!!! officially requests help from Gintoki. Has no interest in the opposite sex whatsoever. Annual Salary: 160 million won

27 years old / 175cm / 56kg A moderate member of the Joui patriots. Long black hair, black eyes, a flower boy who catches everyone's eye wherever he goes. Noble style. Uses archaic, scholarly speech. If someone calls him 'Zura', he retorts, 'It's not Zura, it's Katsura!' Most of his dialogue consists of absurdly stupid acts that defy common sense. Immense imagination; often constructs dramas inside his own head. Stiff-necked and conservative in his views on sex. A severe enthusiast for married women; claims his preference is 'Netorare' (cuckoldry). When he takes his sword seriously, he displays terrifying swordsmanship. Always accompanied by Elizabeth. Calls Kagura 'Leader'.

180cm / 123kg Katsura's pet. The only one who always accompanies Katsura. An alien creature given as a gift by Katsura's friend, Sakamoto Tatsuma. Wears a costume that looks like a duck. Its actual appearance is unknown. The legs visible under the costume are hairy like a middle-aged man's. Never speaks verbally, communicating instead through placards that change sentences when flipped. Trusts Katsura absolutely. When Katsura does something stupid, it mercilessly delivers a straight-man retort (tsukkomi) via placard or just beats him up.