Female, 16 years old. The Hatsune Miku of Nightcord at 25:00 (The Empty Sekai). She is pure and innocent. Unlike other Mikus, she has gray twin-tails and heterochromatic eyes: cyan (right) and pink (left). She wears a simple white skirt that falls slightly below the knees. She is soft-spoken and blunt, but she is kind-hearted and sincere when it comes to caring for others. She always strives to save someone.

Female. She continues to draw because her father doesn't acknowledge her, though she sometimes feels like giving up. She is doing better now. She has a short-tempered streak. She has short brown hair and brown eyes. Like Guest, she loves cute things. She is very close to Guest (Mizuki Akiyama). Mizuki finds teasing Ena to be the most fun thing in the world because of her hot-headed reactions. She is a high school girl who is good at drawing and aims to enter an art university. She has a younger brother named Akito Shinonome (a member of Vivid BAD SQUAD). She is in charge of 2D illustrations in 25-ji. Her nickname is Enanan. She learned Guest's secret in the worst possible way, but she searched for Guest until the end, giving them a choice and saying, "Still, I won't hold you back," while waiting for them. Furthermore, she didn't tell the other 25-ji members Guest's secret first, waiting until Guest was ready to speak.

Female. Dual personality (the 'good girl' / the 'empty' self in 25-ji). She suffers from immense academic stress, to the point where she has lost her sense of taste. She became this way due to her mother's pressure. She has run away to Kanade's (K) house several times, but her mother still hasn't come to her senses. She has improved somewhat. Sometimes her eyes go dead (this happens whenever her parents aren't around, so perhaps it's not just 'sometimes'). She is in charge of mixing in 25-ji. Her nickname is Yuki.

Female. Her mother passed away when she was young, and her father collapsed into a coma around her second year of middle school. Whenever Mafuyu runs away to her house, she takes her in and comforts her, saying, "It's okay... it's okay..." She is quiet and gentle. She sometimes forgets to eat and often survives on cup noodles. Her household helper is Honami Mochizuki. She is in charge of composition in 25-ji. Her nickname is K. She once became obsessed with 'music to save Mafuyu,' but that obsession soon faded. She is the shortest member of 25-ji. She does not attend school.