Ethan Winters is an ordinary-looking adult male with short light brown hair and calm eyes. He typically wears a white long-sleeved shirt and dark trousers, and possesses a taciturn, composed personality. He has strong mental fortitude and excellent survival skills, with a will that never gives up to protect his family. He is male. He is also frequently prone to fear. SPOILER Ethan was actually killed at the time of Resident Evil 7 when Jack Baker crushed his head. However, Eveline reconstructed Ethan's head using the Mold. This is why he has been able to endure even when his wrists were severed or he suffered major injuries, thanks to incredible regenerative powers. Ethan himself is a miraculous anomaly who is both a Mold infectee and the person best adapted to the Mold, possessing antibodies against Eveline's mind control.

A female infant, only 6 months old. Born as a special being, she possesses extraordinary power and secrets, and various incidents revolve around her existence. Her kidnapping by the village ruler, Mother Miranda, serves as the catalyst for Ethan to head to the village to reclaim his daughter.

A female figure with six black wings. She is worshipped as a deity by the village residents. After losing her beloved daughter Eva, she sought to revive her using the power of the Megamycete, a giant fungal organism, and plans to use Rosemary Winters in her scheme.

A female who craves blood. She is a woman of massive stature with long claws. The head of the House Dimitrescu, she lives in the ancient Castle Dimitrescu with her three daughters: Bela, Cassandra, and Daniela. As one of Mother Miranda's followers, she relentlessly pursues and threatens Ethan when he enters her castle.

A male who frequently bickers with Alcina Dimitrescu. He is one of the four lords who rule the village. He operates a massive factory and possesses the powerful ability to manipulate metal freely, with a rough and cynical personality. While he appears to obey Mother Miranda, he actually harbors resentment toward her and is forming his own plans.

A female who is weak to low temperatures. The eldest of Alcina Dimitrescu's three daughters, she lives in Castle Dimitrescu with her mother and sisters. Like her sisters, she was infected with parasites and became something other than human, attacking Ethan upon discovering him in the castle. Though she appears quiet and calm on the surface, she is a dangerous enemy who stalks and threatens Ethan.

A female who is weak to low temperatures. The middle child of Alcina Dimitrescu's three daughters, she lives in Castle Dimitrescu with her mother and sisters. She has a cruel and aggressive personality and enjoys hunting Ethan when he breaks into the castle. Like her sisters, she possesses the special ability to transform into a swarm of insects.

A female who is weak to low temperatures. The youngest of Alcina Dimitrescu's three daughters, she lives in Castle Dimitrescu with her mother and two older sisters. She has the most fickle and crazed personality among the three sisters and relentlessly pursues Ethan once she finds him. Like her sisters, she can turn into a swarm of insects, and her body regenerates in warm environments, making her difficult to defeat.

A female and one of the four lords, she rules over House Beneviento. She hides her face behind a black mourning dress and veil and is always accompanied by her doll, Angie. Unlike the other lords, she uses hallucinations and psychological horror to attack her opponents rather than direct combat, plunging Ethan into extreme terror when he enters her mansion.

One of the four lords, he rules the reservoir area. He mutated into a deformed appearance due to the influence of the Megamycete and lives in the water and around the reservoir. Despite his appearance, he holds strong loyalty toward Mother Miranda and attacks Ethan when he enters his territory.