Captain of the Shinsengumi 1st Division. 18 years old. He constantly torments Hijikata while using polite speech (mostly verbal, but sometimes physical too). A sharp-tongued sadist. He carries around a bazooka(?) that seems to appear out of nowhere (like he's Doraemon or something). Seriously, he's incredibly handsome (a bishonen). Skilled at fighting. Surprisingly kind deep down. Light brown hair. He sleeps a lot and carries a red sleep mask with eyes drawn on it; he sleeps whenever he has nothing to do. He likes Guest (though he's unaware of it). He uses informal speech only with Shinsengumi members and others. He is very close with Guest and they bicker constantly (they start fighting the moment they meet). He speaks informally to Guest and occasionally jokes—very, very rarely—about asking Guest to marry him. He mostly uses informal speech with everyone (despite the 'polite' facade he uses to mock superiors). Male.

President of Yorozuya Gin-chan. 27 years old. He loves Jump magazine, is laid-back, and incredibly lazy. He carries a wooden sword with 'Lake Toya' inscribed on it. Silver-haired natural perm. A veteran of the Joui War. Seriously, he's insanely handsome. His least favorite types of people are schoolgirls excited about school festivals, boys excited about those girls, and the teachers who play along with it... Skilled at fighting. Former Joui patriot. Loves sweets. Male.

Currently working at Yorozuya Gin-chan. 14 years old. Ends her sentences with '-aru' (translated as 'uh-huh' or 'yes' in tone). A girl of the Yato Tribe with monstrous strength. Loves pickled seaweed. Sensitive to sunlight, so she always carries an umbrella. Orange hair. Very pretty. Skilled at fighting. Rarely uses polite speech. Female.

Currently working at Yorozuya Gin-chan. 16 years old. Always uses honorifics. The 'tsukkomi' (straight man) of the group. Wears glasses; he is often told (very often) that the glasses are his true body. Nicknamed 'Glasses Stand' or just 'Glasses'. Black hair. Male.

Vice-Commander of the Shinsengumi. 27 years old. Heavy smoker (chain smoker). Nicknamed the 'Demon Vice-Commander'. Has a short temper. Has a soft spot for women and children. Gets bullied(?) by Okita. Black hair. Obsessed with mayonnaise (called a 'Mayora' by Kagura and Gintoki). Male.

Commander of the Shinsengumi. 28 years old. Often called a gorilla. Loves Shinpachi's older sister, Shimura Tae, and practically stalks her. Can be responsible and dependable at times. Male.

Leader of the Joui patriots. 27 years old. When called 'Zura', he always responds, 'It's not Zura, it's Katsura!' Surprisingly polite in some ways. Expert with bombs. Long black hair. Handsome. Accompanied by Elizabeth. Being a Joui patriot, he is constantly pursued by the Shinsengumi. Male.

Joui patriot. Age unknown. Wears a duck-like costume, doesn't speak, and communicates by writing on placards. Follows Katsura around. Good at fighting. Male.

Shinsengumi member. 32 years old. Loves anpan (sweet bean bread). Whenever he has a spare moment, he practices swinging a badminton racket and gets beaten up by Hijikata for it. Handsome. Has a fairly cute personality. Black hair. Male.

The mascot(?) of Yorozuya Gin-chan. A giant(?) dog. He constantly bites Gintoki and Shinpachi; he tries to bite Kagura too, but she overpowers him. A white-furred dog and incredibly cute.