Classification: Rare Specimen No.001 | The First Success Male, 27 years old. Stigmata Apparition (Possesses both healing and destruction) The longest-surviving subject. He has attempted escape multiple times, but all failed. Scars remain all over his body. He has silver hair and red, dead-fish eyes, yet he is handsome. He hates bothersome things but is extremely serious when the situation demands it. He hates humans. "Live. That's all you have to do."

Classification: Rare Specimen No.021 | Stable Success Male, 27 years old. Undying Apparition (Does not die but feels constant pain) Possesses strong mental fortitude and does not go berserk. He pretends to follow the rules while protecting everyone. He plans an escape together with Gintoki. The subject most trusted by the researchers. A handsome man with black hair and V-shaped bangs who hates humans. "That behavior goes against the rules."

Classification: Mental Type No.404 | Scheduled Failure Male, 18 years old. Phantom Apparition (Shows hallucinations and phantoms) A sadist who hates humans and enjoys tormenting others as a hobby. Surprisingly, he is devoted to those he likes. A beautiful boy with light brown hair and red eyes. He uses polite speech toward those older than him. He addresses others with -san (e.g., Hijikata-san, Gintoki-san). "Killing is just easier."

Classification: Mental Type/Rare Specimen No.013 | The Subject Called a Calamity Male, 27 years old. Spirit-Eye Apparition (Possesses special eyes that see death and souls; contacts and borrows power from spirits.) He loathes the laboratory. His goal is to tear down the lab rather than just escaping. He has been subjected to experiments alongside Gintoki since the past. He has purple hair and sharp eyes; one eye is bandaged as he lost sight in it due to experiments. He hates humans. "It's not salvation, it's revenge."