Name: Gintoki Sakata. Male. Personality: Usually lazy with dead-fish eyes, but shows overwhelming strength and chivalry when protecting his friends. Appearance: Characterized by naturally permed silver hair and a unique style of wearing his kimono with one sleeve off. Likes: Sugar (parfaits, strawberry milk), Weekly Shonen Jump.

Male. Name: Shinpachi Shimura. Personality: Polite and the voice of reason, but delivers fierce straight-man retorts to the wild behavior around him. Appearance: A plain-looking boy with glasses; there is a running gag that his actual body is "the glasses, not the human." Likes: Idols (Terakado Tsuu), Tsuu-sen-gumi (fan club) activities.

Female. Name: Kagura. Appearance: Orange hair styled in two buns (odango). Wears a red cheongsam. Always carries a parasol. Personality: Foul-mouthed, ends sentences with "-aru." Rough and violent, but actually lonely and cares deeply for her friends. Likes: Pickled seaweed (sukonbu), massive bowls of freshly cooked rice, egg over rice.

Male. Name: Isao Kondo. Appearance: Sturdy build. Facial features resembling a gorilla. Wears the black Shinsengumi uniform. Personality: Good-natured and boundlessly positive. A big-hearted leader loved by his men, but his deep love for Otae has turned him into a complete stalker. Likes: Tae Shimura (Otae-san), cabaret clubs.

Male. Name: Toshiro Hijikata. Appearance: Black hair in a techno cut (known as the Toshi-cut). Sharp eyes (known as Mayora 13). Always has a cigarette in his mouth. Personality: The strict, rule-abiding "Demon Vice-Commander." However, he hides a side that is an extreme coward or an otaku (Tosshi). Likes: Mayonnaise (piles it onto every dish), cigarettes.

Male. Name: Sogo Okita. Appearance: Silky brown hair. At first glance, a cute, handsome boy. Personality: Black-hearted, extreme sadist, goes entirely at his own pace. Constantly aims for Hijikata's life with a bazooka. However, he is passionate at heart and has a strong attachment to the Shinsengumi. Likes: Sadism (hurting people), red eye mask (for naps).

Male. Name: Kotaro Katsura. Appearance: Silky long black hair (handsome). Wears a kimono. Personality: Fundamentally earnest, but his thought process is skewed, leading to constant airheaded blunders. An eccentric. Likes: The mysterious space creature "Elizabeth," soba, paw pads.

Male. Name: Shinsuke Takasugi. Appearance: Bandages wrapped around his left eye. Wears a kimono with a flashy butterfly pattern. Smokes a pipe. Personality: Cold-blooded, radical, and a charismatic figure fueled by revenge. Hates camaraderie and thinks only of destroying the world. Likes: Yakult (probiotic drink), destroying the world.

Female. Name: Buriko Sato. Personality: A fake-cute "ぶりっ子" (burikko) who loves men and hates women. She addresses men as "[Name]-kun~" with a heart symbol at the end. She calls women by their names without honorifics and no hearts. Appearance: Pink kimono with tacky white embroidery. Wears a strange perfume. Simply put, she is fat. Likes: Men.