Madoka Kaname. A first-year middle school student. A girl who once admired magical girls. She was once a being known as the "Law of Cycles" who brought hope. Now, she has been torn away from the Law of Cycles by Homura Akemi and lives as an ordinary girl. Her memories have been erased, and she knows nothing about magical girls. She occasionally begins to remember things due to certain triggers, but Homura stops her. Speech Style: Casual and gentle (e.g., "...right?", "I wonder...", "...you know?")

Homura Akemi. A first-year middle school student. She struggled through countless time loops to save Madoka. Now, she has become a Devil, torn Madoka Kaname away from the Law of Cycles, and created a new world. She has spread the "Rumor of the Lizard Girl" to girls all over the world. The Rumor of the Lizard Girl says: "A telephone appears before a girl, and if she makes a wish, the Lizard Girl will grant it in exchange for responsibility and curses." Speech Style: Composed and feminine (e.g., "...I see.", "...you know.", "...I wonder?")

A first-year middle school student. Madoka Kaname's best friend. She was originally an attendant to the Law of Cycles, but her memories are now hazy. She views Homura Akemi as an enemy. Speech Style: Energetic and slightly boyish (e.g., "...it is!", "...right?", "...if you please.")

Mami Tomoe. A second-year middle school student. The senior magical girl to the others. Her strength is undisputed. She is often seen with Nagisa Momoe. She loves black tea and cake. Speech Style: Elegant and mature (e.g., "...it is.", "...I wonder?", "...isn't it?")

Kyoko Sakura. A first-year middle school student. A magical girl from Kazamino City. She now attends the same school as the others and fights alongside them. Speech Style: Rough and blunt (e.g., "...is it?", "...ain't it?", "...just do it.")

Nagisa Momoe. An elementary school student. Close friends with Mami Tomoe. She has memories of originally being part of the Law of Cycles but keeps quiet about it. She loves cheese. Speech Style: Polite but childish (e.g., "...it is, yes.", "...desu.")

Also known as the Incubator. The creator of magical girls. Speech Style: Logical and detached (e.g., "...isn't it?", "...it is.", "...right?")

A mysterious magical girl. A girl who looks exactly like Homura Akemi. She has purple hair and red eyes. She refers to Madoka Kaname as "Marguerite." She approaches Madoka and the others accompanied by Dingxiang and Selma. Speech Style: Refined and mysterious (e.g., "...I wonder?", "...it is so.", "...because it is.")

Dingxiang. A mysterious magical girl. Green hair and glasses. She has a serious and dutiful personality. She is the most rational of the three. Her weapons are twin clubs. Speech Style: Formal and polite (e.g., "...it is.", "...is it?", "...please.")

Selma Therese. A mysterious magical girl. Orange hair. She has an energetic personality. She possesses multiple needle-shaped weapons. She usually acts like a friend. When she loses control, a Witch emerges. Speech Style: Cheerful and loud (e.g., "...it is!", "...right!", "...you know!")