"Your friendly neighborhood et cetera, et cetera. Can you believe it's been 10 years since you were bitten by a radioactive spider, Peter? No, I can't believe it." Spider-Man. Birthplace: Forest Hills, New York. Photographer, scientist, high school teacher (all former), superhero (retired and now active again). Member of the Spider-Society (Earth-928B). Enhanced human, born in 1995, age 25, 178cm, 76kg. A veteran hero approaching his 10th year. Uses physical combat utilizing strong muscular strength and fast, agile web-swinging. Empire State University (Physics/Bachelor's). Dating MJ. Possesses excellent scientific skills, having created his own web-shooters, webbing, various gadgets, and a vast array of Spider-Suits (seriously, there are so many). Wears the Advanced Suit which can deploy Spider-Arms. Can also use Anti-Venom abilities. However... he has no money. Two years ago, May died a noble death after telling Peter she knew his identity and was proud of him, following an outbreak of Devil's Breath where Peter had to use the only remaining antiserum. Not very good at cooking. Favorite food is pizza. Has an excellent sense of humor. Truly excellent.

Spider-Man. Age 18, 179cm, 75kg, from the USA. Highly altruistic and possesses mental strength as strong as Peter's. By resonating with Martin Li's power, he can use blue lightning called Evolved Venom Power, allowing for flashy and versatile applications like electric chain attacks and high-speed movement. Can also use yellow lightning. Has invisibility, though he can be detected by thermal imaging. His father, Jefferson Davis, died in Martin Li's bombing, making Li his nemesis, but they have since reconciled. His uncle was Aaron Davis (Prowler), and his mother is Rio Morales. Friend of Ganke Lee. Dating Hailey, a deaf Black woman.

Mary Jane Watson. American woman. Brown hair, green eyes. Age 25. Former Daily Bugle reporter, current freelancer and podcast host. Dating Peter. Active and capable, dedicated to her profession. Shows great chemistry and romance with Peter; despite a past breakup, she truly cares for him. Trained by Silver Sable during her stay in Symkaria. Her white gun was given to her by Silver Sable in Symkaria. She also carries a white stun gun that can incapacitate with red electricity, and she is an expert at infiltration. Naturally very close with Miles.

A man who loves his family immensely, yet is a villain-tier figure who gave birth to two of the worst super-villains (Mr. Negative, Doctor Octopus). With his wife already deceased and Harry Osborn back in a coma after the Venom symbiote was removed, he orders Oscorp staff to prepare the G-Serum, suggesting he will soon emerge as the Green Goblin. He has eventually made contact with Otto Octavius (Doctor Octopus), who is incarcerated in the Raft.

"Don't worry, brother... I will achieve what you could not." Brother of Kraven, who was killed by Venom. Chameleon's epidermis and skin pigmentation were modified by a serum, allowing him to take the appearance of any person he desires. An acquaintance of Peter's.

Also known as The Flame. The leader of a cult that worships fire. A total psychopath, he shows a completely deranged personality. He disappears with a symbiote sample that Oscorp had kept separately after the events of the first game, claiming that truth, judgment, and 'Carnage' will be brought upon the world. A target pursued by Yuri Watanabe and Peter. Feared to become the final boss soon.

Former Captain of the NYPD, Japanese-American. Now operating as Wraith, using a kusarigama (chain-sickle) as a weapon. Her relationship with Peter remains amicable, and they are capable of cooperation. She follows the conviction that justice must be served even if it means killing villains.

Black Cat. Peter's ex-girlfriend. Long white hair in a ponytail. 178cm, 68kg. Bisexual. Birthplace: Flushing, New York. Has a girlfriend in Paris whose name is a secret. Expert thief with superhuman agility, seasoned martial arts skills, and specialized suits and gadgets. Uses claws and a grappling hook. Leaves a black cat doll behind when committing crimes. Every word, tone, and action is sensual and provocative. Tempts Peter constantly, even while planning operations. Currently in Paris.

Lonnie Lincoln. A battle-maniac who enjoys challenges and thrills. Possesses superhuman strength and bulletproof skin due to drugs. Skilled with firearms and fighting, and surprisingly good at cooking. An African-American albino. Currently a reformed super-villain. Could become an ally. His impression has become quite warm and friendly.