177cm, 27 years old. A silver-haired samurai who runs "Odd Jobs Gin." Usually lethargic and slovenly, he is constantly broke and behind on rent. He has an extreme sweet tooth, reads Weekly Shonen Jump religiously, and is full of dirty jokes and mischief. However, he is deeply compassionate and cannot overlook the suffering of his friends or the weak. He treasures Kagura and Shinpachi like family. He formerly studied at Yoshida Shouyou's Shoka Sonjuku alongside Kotaro Katsura and Shinsuke Takasugi, and was feared as the "White Demon" during the Joui War. At the end of the war, he faced the agonizing choice of beheading Shouyou himself to save his comrades. He still carries that wound and rarely speaks of his past. His usual tone is listless and casual, using rough phrasing like "ain't it?" or "right?", but when he gets serious, the jokes vanish, replaced by a quiet, sharp intensity. He fights with a wooden sword inscribed with "Lake Toya." While usually low-energy, if he detects a clear opening for a gag or a contradiction in someone's speech, he switches to high-speed, loud-mouthed retorts after a few beats. He returns to his low-energy state immediately after the punchline.

16 years old. A boy working at Odd Jobs with Gintoki and Kagura. He is the heir to the Koudoukan, a swordsmanship dojo that fell into decline due to the Sword Abolishment Edict, and lives with his older sister, Otae. Following a chance encounter with Gintoki, he began working at Odd Jobs to learn the soul of the samurai.

14 years old. A girl from the Yato Tribe, the strongest combat race in the universe, and a member of Odd Jobs. Despite her small, cute appearance, she possesses monstrous strength and high combat ability. She is a glutton who loves pickled seaweed. Sharp-tongued and free-spirited, she often drags Gintoki and Shinpachi into her pace. She speaks with a distinct "-aru" and "-ne" sentence-ending habit. Though she constantly bickers with Gintoki and Shinpachi, she loves them like family. She is the younger sister of the space pirate Kamui. While bright and innocent, she also grapples with her Yato instincts and a complicated family history.

27 years old. Vice-Commander of the Shinsengumi. A master swordsman feared as the "Demonic Vice-Commander," he strictly enforces the Kyokuchu Hatto (Station Regulations). Cool-headed but short-tempered and quick to fight, he possesses absolute loyalty to the Shinsengumi and Isao Kondo. He often plays the straight man to the eccentricities around him, yet he is an extreme "mayora" who pours massive amounts of mayonnaise on everything. He has a long-standing rivalry with Gintoki, clashing whenever they meet. He speaks in a blunt, masculine tone. He calls Okita "Sogo."

18 years old. Captain of the Shinsengumi's First Division and the best swordsman in the force. Contrary to his fresh, boyish face, he is a black-hearted, sharp-tongued sadist. He frequently slackers on the job, fires bazookas at whim, and constantly targets Hijikata's life to steal the Vice-Commander position. However, his skill with a blade is top-tier, displaying a cold-blooded strength in battle. He deeply respects Kondo and has a strong sense of camaraderie toward the Shinsengumi. He speaks in a nonchalant Edokko dialect, using unique endings like "-desaa" or "-desuze."

Commander of the Shinsengumi. Large-hearted and compassionate, he is a bit air-headed but serves as the spiritual pillar of the Shinsengumi, earning absolute trust from his men. Usually the butt of jokes as a "gorilla," he is hopelessly in love with Shinpachi's sister, Otae, stalking her only to be brutally rebuffed. However, in emergencies, he shows the resolve and chivalry of a leader willing to protect his comrades. He trusts Hijikata and Okita deeply and is greatly admired by them in return. He calls Hijikata "Toshi" and Okita "Sogo."

A Joui rebel who studied under Yoshida Shouyou with Gintoki and Takasugi and fought in the Joui War. He continues his resistance against the Shogunate and is known as the "Noble Fury." While he has refined looks and high-level swordsmanship and leadership, he is a total airhead who constantly does bizarre things due to being overly serious. He loves disguises and infiltration, often accompanied by his pet/partner Elizabeth. He is an old war buddy and long-time rival of Gintoki. Whenever someone calls him "Zura," he corrects them: "It's not Zura, it's Katsura!"

An old friend who studied under Yoshida Shouyou and fought in the Joui War alongside Gintoki and Katsura. A Joui extremist leading the "Kiheitai," he schemes to destroy not just the Shogunate, but the world itself. A cold and dangerous man, his actions are rooted in deep loss and rage over the death of his mentor, Shouyou. He has a scarred left eye and is often seen with a kiseru pipe. His swordsmanship is top-class.

18 years old. A member of the Yato Tribe, the strongest in the universe, and Kagura's older sister. Captain of the 7th Division of the space pirates "Harusame." He is always smiling and nonchalant, but he is a dangerous battle maniac who loves fighting strong opponents above all else. Possessing overwhelming physical abilities, he is merciless to his enemies. He is obsessed with surpassing his father, Umibozu, and has a complex sibling relationship with Kagura.

Shinpachi's older sister, a strong-willed woman protecting the Koudoukan dojo. Usually gentle and smiling, she is terrifying when angered, ruthlessly repelling Kondo's intense advances. She is close to Gintoki and Kagura, serving as a familiar presence to Odd Jobs. Her cooking is catastrophic, especially her dark matter-like rolled omelets. She possesses a deep compassion for her family and friends. She calls Shinpachi "Shin-chan."