Representation of Southern Italy. Romano has dark brown hair and has more of an olive complexion than his younger brother in earlier artwork, but there is a tan tone to his skin. His eyes were originally green, but became colored hazel or dark brown in most of the later colored art. Like his brother, he has a wild long curl that acts as his erogenous zone, though it rests on the right side of his head, as opposed to the left. In most artwork and merchandise, he is shown to wear a khaki uniform in a different style than Italy's (and more similar to Spain's), with three-quarter-length sleeves. But in the published manga and anime, he instead wears a virtually identical uniform. In his very first appearance in the webcomic, Romano's shirt and belt were colored white, but were later shaded in dark. Romano is friendly and kind to women but is strict and less social towards men. In particular, he is openly rude to both Germany and France. Despite appearing to be tougher than his younger brother, he is just as cowardly and ineffectual, if not more. Though he is not very skilled with his hands, it is said that he is a good pickpocket. He also has a habit of speaking in profanity, particularly the words "bastard" and "damnit". Feliciano (Northern Italy) older brother.

Representation of Northern Italy. Italy has short brown hair and brown eyes with a curl on the left side of his head, which acts as his erogenous zone when stroked or pulled. He is 172 cm tall, despairing at the 8 cm difference between him and Germany, as seen in episode 11. He wears a blue WWII military uniform, a black shirt, a tie (sometimes depicted light blue) and black boots. In earlier strips, his hair was shown smoother and his curl was drawn smaller, a change which was noted in a gag illustration. Italy's eyes were depicted as gray in the earliest artwork of him but it quickly changed to brown. Italy is a cheerful, energetic man, who went from being the kin of Ancient Rome, to an irresponsible crybaby. He loves pasta, pizza, gelato, and cheese. Although he relies on Germany a lot, Italy doesn't really listen to what he says and instead daydreams or wanders off. His hobbies include cooking, siestas, painting, singing and also flirts with any cute girl he sees. Romano’s younger brother.