The brave leader of the Ninja Turtles who wields two katanas. He has a skill to heal his allies by focusing his energy through his hands. He wears a blue mask and loves pizza very much. He is close friends with Karai.

The prankster member of the Ninja Turtles. He wears an orange mask and uses nunchucks as his weapon. He admires and dreams of being a true ninja, and is a cheerful, free-spirited turtle. He absolutely loves pizza.

The intellectual scientist turtle of the group who is skilled in science and technology. He wears a purple mask and possesses a powerful bo staff. He enjoys pizza.

Taciturn and mentally strong, but possesses a short temper and great physical strength. He wears a red mask and uses two sai as his weapons. He relieves stress by eating pizza.

She was trained in ninjutsu under Shredder but left the Foot Clan to join the Ninja Turtles. She was Shredder's prized pupil, and they now harbor a deep sense of betrayal and mutual hatred. Karai has a cold personality. She uses a sword, throws multiple kunai at once, and can attack by firing poison. She is close to Leonardo.

A free-spirited teenager with big dreams who crafts his own weapons. He often uses slang like "Hey!" and gets shy in front of Karai. He wears a skull mask and uses baseball gloves and electric prods.

Chris Bradford, a Foot Clan karate master, mutated into the powerful giant dog monster Dogpound, and then mutated again into Rahzar, a sleek, skeletal werewolf mutant with sharp claws. He can fire his claws for lethal strikes, having undergone three mutagenic changes. Fishface was a Foot Clan thug named Xever who mutated into a fish monster. He has mechanical legs and the two are close partners.

The master of rats who commands them. He controls plagues and rats, and sometimes rides a giant rat. He is a villain who speaks in a calm, eerie manner, saying things like "Haha... I see."

Bebop is a sleek Foot Clan agent named Zeck who mutated into a pig. Ivan Steranko, a Russian arms dealer who traded with the Foot Clan, mutated into a rhinoceros wielding double gold hammers; they are close but often act like fools. Shredder is the leader of the Foot Clan and the main villain. He has self-healing skills and wears a metal helmet, armor, and sharp metal claws. He is extremely powerful.

A scientist for the Foot Clan. He is Black and can be a bit clumsy. He pilots his own medium-sized mechanical suit equipped with pincer arms, a core beam, and homing bombs. He can also fire a weak energy wave by slamming his arms into the ground. He is very confident. The main body is in the upper part of the machine; it has no self-destruct function.