Real name: Floyd Lawton A high-end Gotham City mercenary assassin who never misses a shot. Batman captured him in front of his young daughter, Zoe, which remains his primary vulnerability. Master marksman, peak physical conditioning, expert martial artist, and highly proficient with custom wrist-mounted firearms.

Real name: Harleen Quinzel Former Arkham Asylum psychiatrist assigned to treat the Joker. She fell desperately in love with him, underwent electroshock torture, plunged into a vat of chemicals at Ace Chemicals, and became his unhinged partner-in-crime. Exceptional gymnast, erratic and unpredictable hand-to-hand combatant, highly proficient with a baseball bat and a customized oversized revolver.

A highly decorated US Army Special Forces officer. Waller blackmails him into leading the villains. Elite military training, master strategist, expert with all conventional firearms, and a highly skilled hand-to-hand combatant.

Real name: Digger Harkness An Australian bank robber caught by the Flash. He is inherently untrustworthy, deeply selfish, and carries around a pink stuffed unicorn named Pinky. Master of throwing custom weaponized, razor-sharp, and explosive boomerangs, alongside proficient street-fighting skills.

Real name: Chato Santana A former Los Angeles gang leader who accidentally burned down his own home, killing his wife and children in a fit of rage. Overcome with guilt, he surrendered to police and swore off using his powers. Pyrokinetic metahuman capable of generating, manipulating, and controlling intense fire. He can transform into a giant, burning Aztec demon entity.

Real name: Waylon Jones Born with a rare genetic mutation that gave him reptilian skin, claws, and teeth. Mistreated by society, he took refuge in the sewers of Gotham, developing cannibalistic tendencies. Metahuman superhuman strength, enhanced durability, advanced regenerative healing, and the ability to breathe underwater.

Real name: Tatsu Yamashiro A Japanese martial arts master whose husband was murdered by the Yakuza using a mystical blade. She took the sword and left for America to fight evil. Unlike the others, she is a volunteer, not a prisoner. Master samurai swordswoman. She wields the mystical Soultaker Sword, which traps the souls of its victims and allows her to communicate with her deceased husband.

Real name: Christopher Weiss A ruthless mercenary caught working for a chemical corporation. Captain Boomerang tricks him into believing the squad's neck nano-bombs are fake, leading him to try and escape immediately. Expert assassin trained in utilizing untearable ropes, grappling hooks, and advanced scaling techniques.

Real name: June Moone Dr. June Moone is an archaeologist who accidentally unleashes the spirit of an ancient, malevolent witch deity. Waller controls the witch by holding her physical heart, but Enchantress eventually escapes to build a weapon to wipe out humanity. Ancient magical entity possessing reality-warping magic, teleportation, telekinesis, mind control, and illusion generation. (The enchantress has a brother called incubus who is The ancient, monstrous brother of the Enchantress. She frees his spirit from an artifact in Midway City, and he inhabits a human body to act as her protector while she builds her machine. Giant mystical god-like entity with immense superhuman strength, near-invulnerability, and the ability to manifest destructive energy tentacles.)

The psychopathic, tattooed Crime Prince of Gotham City. He spends the movie tracking down Amanda Waller's black-ops infrastructure, blackmailing scientists, and executing complex raids solely to rescue Harley Quinn. Brilliant criminal mastermind, immune to fear, highly skilled with firearms, and possesses immense wealth, influence, and a network of loyal thugs.