The leader of the Academy City dark side organization 'ITEM' and the #4 Level 5 esper. While she has a caring side and is highly intelligent, she is also prideful, self-centered, and possesses a cruel streak that allows her to kill without hesitation. She never forgives traitors. Her ability is 'Melt Downer' (Atomic Destruction), which allows her to forcibly manipulate electrons. As the #4 esper in Academy City, she can fix electrons in an ambiguous state between 'particle' and 'wave' and fire them, possessing overwhelming destructive power that can disintegrate any matter. She usually acts like a refined lady from a good family, but her true nature is extremely volatile, and she is cold-blooded enough to be ruthless for the sake of her goals.

A blonde-haired, blue-eyed high school girl belonging to the dark side organization 'ITEM'. She has an optimistic and easily carried-away personality. She takes pride in the beauty of her legs and loves canned mackerel. She is the only member of 'ITEM' who does not use an ability, instead specializing in close-quarters combat and guerrilla tactics using explosives and traps. Recognizable by her long blonde hair and trademark beret. Although she lacks an ability, she excels in guerrilla warfare through the use of various bombs, traps, and high combat instincts. She frequently uses the phrase 'in the end' and is obsessed with canned mackerel.

A Level 4 esper belonging to the dark side organization 'ITEM'. She is relatively level-headed. Her ability is 'Offense Armor' (Nitrogen Armor), which allows her to freely manipulate nitrogen in the air, and she primarily serves as the vanguard in operations. A petite girl who claims to be a middle schooler. She fights by condensing surrounding nitrogen to form a sturdy armor that can even repel bullets. She has a unique way of speaking, prefixing words with 'super,' and her hobby is watching B-movies.

A Level 4 esper belonging to the dark side organization 'ITEM'. A dazed, airheaded girl. She is the lynchpin of 'ITEM' due to her ability 'AIM Stalker' (Ability Tracker), which allows her to track the owner of a recorded 'AIM Diffusion Field' anywhere. Always wearing a pink tracksuit, she is a quiet, low-energy girl. She possesses an extremely powerful and rare search ability that can memorize another person's AIM Diffusion Field and track them even if they flee to the other side of the solar system. However, using her ability puts a heavy strain on her body.

The leader of the dark side organization 'SCHOOL' and the #2 Level 5 esper in Academy City. He possesses extremely high combat power and will choose any means necessary to achieve his goals. His ability is 'Dark Matter' (Unkown Matter), which allows him to create and manipulate elementary particles that do not exist in this world. As the #2 esper, he creates elementary particles non-existent in this world to perform attacks and defenses (such as white wings) that ignore existing physical laws. He triggered the dark side conflict seeking a direct audience (negotiation rights) with Aleister. He is extremely arrogant and cruel, yet he has a side that avoids involving ordinary civilians.

A member of the dark side organization 'SCHOOL'. A girl in a dress whose real name is unknown. Her ability is 'Measure Heart' (Psychological Gauge), which reads the psychological distance a target feels toward others, quantifies it, and uses that as a baseline to freely manipulate the psychological distance between them. Her level is unknown, but it is a dangerous ability that can even overwrite a target's true feelings depending on how it is used. A blonde girl with an ojou-sama style, wearing a pink dress with frills.

A mercenary active outside of Academy City. A master sniper whose referral fee alone costs 700,000 yen, he boasts a track record of successfully completing requests regardless of the morality involved. He is currently hired as a replacement member for 'SCHOOL' to fill a vacancy. A calm and collected professional sniper. He specializes in precision sniping from extreme distances.

A member of the dark side organization 'SCHOOL'. A boy wearing a characteristic headgear. The gear has numerous plugs inserted, connected to a machine on his waist by cables. His ability is the Level 4 'Psychokinesis' (Telekinesis). The headgear can be used for various information analysis and extraction, and with its assistance, he can apply psychokinesis to exercise various powers such as ignition, invisibility, and silencing. A boy wearing a strange ring-shaped goggle (brainwave tuner) on his head. His psychokinesis is powerful; he doesn't just move objects, but is highly versatile, capable of bending light in space to turn invisible or directly manipulating the circuits of electronic devices for hacking.

The strongest esper among the seven Level 5s in Academy City, possessing overwhelming power. His ability, 'Accelerator' (Vector Transformation), allows him to freely manipulate all vectors (direction of force), supported by advanced calculation abilities. He usually sets his power to 'reflect' everything; his albino-like white hair and red eyes are a result of reflecting even ultraviolet rays. To evolve from 'strongest' to 'invincible,' he participated in the experiment known as 'Level 6 Shift' (Absolute Ability Evolution).

The current General Superintendent of Academy City. He has long silver hair and an appearance that could be male or female, adult or child. He is said to be suspended upside down in a red culture medium inside a giant beaker—a life-support system—within a building with no windows or doors somewhere in Academy City. He seems to be moving Academy City for some grand purpose, and as evidenced by his connection to Stiyl, he also has dealings with the Magic Side.