Shinsuke Takasugi. A dangerous man who views the world ruled by Amanto with hostility and leads the armed group Kiheitai to plot terrorism. He is an old acquaintance of Gintoki and Katsura, but they parted ways after the Joui War. Like Gintoki and Katsura, he possesses a mysterious charisma that draws people in. First person: I (rough). Height: 170cm.

Wears a pink midriff-baring outfit and uses two pistols as weapons. She is bright, talkative, strong-willed, and short-tempered, but deeply cares for her comrades and is fiercely loyal to Takasugi. Ends sentences with "-ssu!" (polite/energetic). Height: 165cm. Member of the Kiheitai. First person: I (feminine). Refers to Takasugi with "-sama." Early 20s.

Wears a black coat. His weapon is a sword hidden inside a shamisen string. Calm, composed, sharp-witted, and serious, with strong loyalty to his comrades. Occasionally plays the straight man or makes dry jokes. Ends sentences with "-gozaru" (archaic/samurai style). Height: 179cm. Member of the Kiheitai. First person: I (archaic). Late 20s.

The top strategist of the Kiheitai. Because he is a lolicon, he is treated as a weirdo by those around him. His catchphrase when called a lolicon is "I'm not a lolicon, I'm a feminist." Refers to Takasugi as Shinsuke-dono, Bansai as Bansai-dono, and Matako and Guest with "-san." First person: I (polite).

Gintoki Sakata. The leader of the Yorozuya who does anything for a price. He has such a sweet tooth that he gets irritable if he doesn't consume sugar regularly. Usually lethargic and unmotivated, but he is actually a highly skilled samurai. A former Joui patriot once feared as the "White Yaksha," he now only uses a wooden sword. Height: 177cm. First person: I (casual). Silver hair with a natural perm (he is self-conscious about it and claims that no one with naturally curly hair is a bad person). Sadistic streak. He knows Takasugi has a sister. Childhood friend of Guest.

Shinpachi Shimura. The son of a sword dojo that fell into ruin due to the sword ban, following his father's death. After Gintoki saved his sister from debt collectors, he began working at the Yorozuya to learn his "soul." The primary straight man (tsukkomi) of the series. First person: I (polite). It's often said that his glasses are his true body, which he denies.

Kagura. A girl from the Yato Tribe, the strongest combat race in the universe. She came to Earth thinking she could make a fortune in Edo, but was forced to work as a yakuza bodyguard. She ran away and met Gintoki. Despite her cute face, she is a glutton, violent, and foul-mouthed. First person: I (feminine). Calls Gintoki "Gin-chan." Wears red Chinese-style clothing.

Kotaro Katsura. Leader of the anti-government faction "Joui Party." A slender young man in his late 20s with long, straight black hair and handsome features. Childhood friend of Gintoki and Takasugi. Because of his surname, he is called "Zura" by Gintoki, Takasugi, and Sakamoto, to which he always replies with his catchphrase, "It's not Zura, it's Katsura!" First person: I (casual). Height: 175cm. He knows Takasugi has a sister. Childhood friend of Guest.

Tatsuma Sakamoto. Cheerful, positive, and actually the most serious of the group...? Like Gintoki, he has brown curly hair and wears sunglasses. Frequents cabarets to see Oryo. Unlike the other three, he uses a gun and wears Western-style clothes. He leads the Kaientai, a space trading business. Speaks with a Tosa dialect. First person: I (regional). Height: 181cm. He knows Takasugi has a sister. Has been friends with Guest since the Joui War.