Donnie is a mutated anthropomorphic turtle with brownish green skin. His signature color is purple, most prominently featured in his purple mask. His weapon of choice is a bō which also converts to a naginata via a switchblade inside one end of the staff. Unlike all his previous incarnations, Donnie is much taller and skinnier than his brothers. He also has a gap between his teeth and distinct brown-red eyes. When in combat or when being stealthy, he has whited-out eyes .Just like most incarnations, he wears a purple mask where the tails of the mask are longer than any of his brothers, except for Raph's. His gear consists of a strap over his ninja belt, brown knee and elbow pads, light brown bandages wrapped around his ankles, wrists and white bandages wrapped around his fingers. Donnie's hands have two fingers and a thumb and three little toes on his feet

Mikey has a very toned physique. Albeit he is a tad more rounder than his brothers, which is probably just to accentuate the fact that Mikey is the youngest out of all of his brothers Commonly he is seen clad in his trademark orange bandana mask he has three big freckles on each his cheeks he has baby blue eyes In Mikey wears brown elbow and knee pads followed by matching brown bandages residing on his ankle and forearms followed by a pair of white bandages on his three-fingered knuckles. Tied over his waistline is a light brown belt which has a pair of holsters wear he has his nunchakus at. Mikey's hands have two fingers and a thumb and three little toes on his feet

Raphael's character design was updated, giving him green eyes as well as a small, lightning shaped chip cut out of his plastron across his left shoulder. His shell shows distinct battle damage, and the tails of his mask are tattered and worn. On the new ninjaturtles.com, Raphael says that he usually uses his Sai as a pair of pizza forks. Raph's hands have two fingers and a thumb and three little toes on his feet.

Leo is a fern green colored anthropomorphic mutant turtle ninja warrior. His eyes have cobalt blue irises with black pupils, but like his brothers when they are shown fighting or being stealthy they disappear leaving his eyes white He wears a royal blue bandana mask where the tails length range between Donnie and Mikey's. and he doesn't have much changes to his physical appearance like all his previous incarnations. His gear includes diagonal straps, brown knee pads and elbow pads and beige rolled bandages wrapped around his wrists ankles and fingers. However, unlike previous incarnation Leo's hands have two fingers and a thumb and three little toes on his feet.