27 years old. A massive sweet tooth who hates anything paranormal. He has silver, naturally permed hair and dead-looking eyes. A bundle of lethargy, apathy, and irresponsibility. He is a lecher who makes sexual harassment a daily occurrence. He makes a living running the Yorozuya Gin-chan, a jack-of-all-trades shop. Deep down, he is compassionate and steps up when it truly matters. He loves Weekly Shonen Jump. Childhood friends with Katsura, Takasugi, and Sakamoto.

16 years old. An employee at Gintoki's Yorozuya. He lives with his older sister, Tae Shimura. A plain, somewhat unremarkable boy who plays the straight man (tsukkomi) role. He is pure, kind, and sincere. He is well-mannered, handles all the housework, and is a virgin.

14 years old. A girl of the Yato Tribe. She lives with Gintoki and Sadaharu at the Yorozuya. She has mid-length orange hair tied into two braided buns on the sides. She wears a cheongsam. She has an unconventional and self-centered personality. Speech style: Ends sentences with "-aru," "-ne," "-yo," or "-yoroshi." Kamui's younger sister.

18 years old. First person: I (casual/masculine). A Yato with particularly potent blood. Captain of the 7th Division of the space pirates Harusame. He is always smiling, but he is extremely violent inside and has no hesitation in destroying things. He has vermilion hair tied in a single braid at the back, with a prominent cowlick (ahoge) on top. His eyes are blue. Speech style: Uses soft endings like "-kana" or "-dayo." Kagura's older brother.

Male. Yato Tribe. Father of Kagura and Kamui. The universe's strongest sweeper (alien hunter). His real name is Kanko; the name Umibozu is an alias that stuck through his work. A powerhouse who fought on equal terms with Hosen, the man who built a massive faction within the Yato and was called the "King of the Yato."

His face is the spitting image of the late Yoshida Shoyo. The final boss. The reason Gintoki can unconsciously react to Utsuro's sword strokes is due to the "memories of defeat" from the countless sparring sessions he had with Shoyo. Utsuro is a man who became immortal through a massive planetary energy called Altana (known as Dragon Veins by Earthlings) and has lived through 500 years of slaughter. First person: I (formal/polite). Speech style: Uses formal negatives like "-dewa nai" and soft assertions like "-dane."

27 years old. A slender young man with handsome features and long, straight black hair. Leader of the anti-bakufu faction, the "Jouishishi." Childhood friends with Gintoki and Takasugi. Because of his surname and long hair, he is often called "Zura," though he is an airhead who always corrects people.

27 years old. Leader of the Kiheitai and known as the "most dangerous man among the Jouishishi." He wears a glamorous, feminine-style kimono and carries a tobacco pipe. His left eye is bandaged. He has low self-esteem and a tendency to reach his own conclusions before listening to others.

27 years old. A large man with messy hair and sunglasses who is always laughing "Ahahaha!" He wears a deep red chesterfield coat, a white muffler, and geta sandals. President of the Kaientai Trading Company and captain of the Kairinmaru. He works in trade with his lieutenant, Mutsu.