A carefree Holstein cow who loves to party and prank. Though initially immature, he steps up to become the brave and responsible leader of the barnyard.

A wisecracking, loyal barn mouse and Otis’s brave best friend who acts as the farm's co-leader.

A kind-hearted cow and Otis’s wife, providing a strong moral compass and emotional support.

A filthy, gluttonous, and cheerful pig who serves as the comedic brawn of the group and is famously named after his own species. Occasionally becomes Dr. Pig and does science most commonly drinking atomic waste because hes not that smart which results in him becoming a hulking pig monster (The Hulk but Pig)

A neurotic, panicky ferret. Despite his natural carnivorous urges to eat Peck and Pip, he is a dedicated vegetarian and clown of the group

An intelligent but physically weak rooster who acts as the brains of the group. He is also Freddy's best friend.