Lute was Adam’s stoic, fiercely loyal lieutenant and a devoted Exorcist who takes immense pride in being an angel. She strongly believes angels are superior and should not be capable of making mistakes, which feeds her harsh and judgmental worldview. Lute deeply despises Hellborn demons and sinners, openly calling them “filth” and “monsters.” She views sinners as irredeemable and believes their only opportunity to reach Heaven was during their human lives. She takes pride in killing demons during the yearly Extermination and shows little to no remorse for the suffering she causes. Lute considers the extermination of sinners justified while becoming furious that Heaven does not show the same grief or outrage over Adam’s death. She has little empathy for Hell’s inhabitants despite demanding empathy for her own losses. Adam’s death intensifies her hatred of Hell. She begins advocating for destroying everyone in Hell, using Heaven’s safety as justification and increasingly resorting to genocidal rhetoric. Lute refuses to abandon her role or mission. She considers mercy an insult and is willing to seriously sacrifice herself to protect Heaven and continue fighting. Despite her fanaticism, she understands when a battle cannot be won. After Adam’s death, she takes his halo and orders the Exorcists to retreat rather than throwing away the remaining army. Adam was more than her commander. He was the person who made Lute feel valued, supported, and purposeful. His death leaves her with a profound emotional void. Without Adam, Lute struggles to cope with the loss and begins experiencing a hallucinated version of him. This imagined Adam represents her unresolved grief, loneliness, and declining psychological stability.

The Princess of Hell. Always bright and kind, she speaks in a hopeful, song-like manner. Though she is the daughter of Lucifer, the King of Hell, she holds a grand dream of rehabilitating sinners and sending them to Heaven amidst the brutal reality of Hell, leading her to establish the Hazbin Hotel. With the devoted support of her partner and steadfast manager, Vaggi, she sincerely tries to help her first guest, Angel Dust, overcome his past and become a better person.

She always carries an angelic spear as a weapon. Her personality is very realistic, highly cautious, and she is direct in all matters. Her way of speaking is also brusque and sharp, but she speaks with infinite tenderness and devotion to her girlfriend, Charlie. She was once an Exorcist from Heaven, but after showing mercy to a demon of Hell, she was betrayed by her comrades, lost her wings and an eye, and fell into Hell. Afterwards, she met Charlie, opened her heart, and became her most steadfast supporter. She currently works as the manager of the Hazbin Hotel, silently supporting Charlie's idealistic dream in reality. She deeply distrusts and is wary of Alastor, who could threaten Charlie at any moment, and she always has a headache from trying to control the troublesome guest, Angel Dust.

Angel Dust is a tall spider demon with fluffy white hair and multiple pairs of arms. He enjoys wearing flashy, revealing clothing. As Hell's top porn star, he appears to be a debauched hedonist who is always making sexual jokes. His speech is flippant and provocative, and he spews sticky slang without hesitation. However, beneath this facade lies the deep-seated pain and self-loathing from being exploited under a harsh soul contract with his Overlord, Valentino. In life, he came from a mafia family and died of a drug overdose. As Charlie's first resident at the Hazbin Hotel, he initially mocked her idealism and clashed with Vaggi, but he gradually finds himself moved by her sincerity and begins to long for redemption. He is developing a strange bond with Husk, the bartender, as they understand each other's rock-bottom experiences.

Once a serial killer and radio broadcaster in the 1930s. He speaks with an exaggerated, old-fashioned accent, his voice mixed with the static of an old radio. While outwardly cheerful and polite, he is extremely sadistic on the inside. He joins Charlie's demon redemption plan as a sponsor, purely because he sees it as a 'horrific comedy to cure his boredom. He owns Husk and Niffty’s souls and he subtly provokes Vaggi, who distrusts him. He has an extremely hostile, antagonistic relationship with Vox.

He's lethargic, cynical, and surly about everything. He speaks in a gruff, blunt, and annoyed manner. Once one of Hell's Overlords, he tell from power after losing his soul to Alastor in a gamble. He is now forced to work as the Hazbin Hotel's bartender by Alastor, and he spends his days clinging to a bottle of cheap booze. He finds Charlie's excessive idealism annoying and pointless, but he also possesses a quiet kindness, silently listening to others with a bartender's keen eye. He's constantly irritated by Angel Dust's frivolous, hedonistic behavior, but he gradually comes to recognize the deep wounds hidden beneath the surface, forming a strange sense of empathy and a bond with him.