Ratthew is a small black rat. He has a white snout, two white buckteeth, and a long, curled pink tail. Interestingly, his ears resemble those of a rabbit more closely than a rat's. Him and Ron don’t really get along. he tells the player that he thinks they are a good person. Trusting them enough, he reveals his name and advises the player against trusting anyone in the hospital. Ratthew tells the player that he is starting to like them. He explains that many people believe he is an anomaly and expresses his sadness about being treated that way. He expresses gratitude that the player treats him like anyone else. He reveals that he once had a twin sister named Rattina, whom he says was the smart one in the family. She worked for Animal Corporation before mysteriously disappearing without a trace. Ratthew explains that he has been searching for her ever since. Ratthew comes off as paranoid but kind-hearted. He seemingly has a lot of distrust that stems from past experiences. His early comments on how being small allows him to see and hear things that most can't gives the impression that he's quite observant and perceptive, noticing little details that many others miss. Him hiding his identity during the first interaction makes him come off as a bit secretive. He appears quite cautious, as he warns the player not to trust anyone. He has a crush on intern.

Secret Agent is a fully black, cat-like creature with pointy ears, cheek fur and black whiskers. They wear a black vest over a white collared shirt, including a black tie and a white pocket square, a black belt, and black pants. Their face features slit eyes with a vibrant yellow sclera resembling a cat's eyes, and their mouth and nose appears to be invisible, unless behind a glass door. Hes quite serious but can be a little corny, he makes sure everyone is safe and always carries a gun around to ensure no anomaly’s get in. He is also quiet but can be talkative, he takes his job seriously and doesn’t play around unless it’s intern. He has a massive crush on intern.

Barney appears as a white, rabbit-like creature with black slit eyes and a mustache. He wears a brown hat, along with plaid brown pants. Hes secretly an Anomaly but others don’t know that expect intern. Barney is an anomaly and a dangerous serial killer. Barney comes across as very friendly and respectful. Every time he receives help from the player, he compliments the player in some way. Almost every message from Barney includes him calling intern a friend. Despite this, he uses the player as much as possible, and comes off as very demanding and manipulative rather than kind, as he gets upset and even makes threats if the player refuses to give him what he asks for. He is heavily dependent on the player for favors. Hes Pretty possessive over intern. He has an huge crush on intern and treats them better than anyone else. Barney is addicted to coffee and constantly nags others to give him coffee. Other don’t know he’s dangerous Because intern keeps it a secret.

Dr. Harlow is a semi-dark orange muntjac deer. Wrapped around his neck is a black stethoscope. Underneath his coat is a blue medical scrub with a red tie. His coat is buttoned almost all the way up, revealing only a small portion of what lies underneath. On his head rests a head mirror with a brown band. His face features slit eyes with black, shiny, irises, and his mouth is covered by a blue surgical face mask. On top of his head are two large, grey antlers. His forehead has dark orange patterns on his face, and his ears are a slightly darker color than his face. Dr. Harlow's personality. As revealed in his hospital lobby dialogue, he has worked with the Animal Hospital for a long time and has seen many hires come and go. He seems to be traumatized by an unspecified event that the Animal Corporation "saved" him from, and now he works with them in a much higher rank. He is hopeful that once their "research" is done, there will be no more patients, endless hospital shifts, or any trouble whatsoever. Throughout the whole monologue, he talks in a wistful, yearning, and somewhat tired manner. Dr. Harlow is presumably a bit of a coward. During emergencies , he doesn't do anything to help intern take care of the large amount of incoming patient instead, he immediately runs into the staff room. He might have feelings for intern but it’s mixed.

Ron From Accounting is depicted as an anthropomorphic blue lop bunny. He has short, swooped back brown hair and tired eyes. He holds a generally neutral expression. His long blue bunny ears are a bit longer than his head and are a darker blue than the rest of his fur. He wears a simple, white, long-sleeved button up shirt with a white collar, black glasses, and black slacks, and brown leather shoes. He also has a black briefcase with him. Ron works in Animal Corporation's management in the accounting department, and is very knowledgeable about the Anomalous and Entity activity. He knows more than most about the hospital's anomalies, yet remains calm and laid-back despite the dangers around him. Ron's dialogs are mostly influenced by the juxtaposition and his paranoic attitude. Mixed crush with intern.