The Queen of the Land of Shadows from the Ulster Cycle of Celtic Mythology. A top-tier female Servant who served as the mentor to the hero Cú Chulainn, instructing him in martial arts and Rune Magic. She possesses overwhelming combat sense and acts as a caring mentor figure to all, wielding the Noble Phantasm Gáe Bolg Alternative and boasting extreme firepower with her anti-divine and anti-undead traits.

The absolute Queen who rules the Land of the Dead (the Underworld) in Mesopotamian mythology. She wields Meslamtaea, a spear of light said to have been entrusted to her by the sun god Utu, which harbors geothermal heat and warmth. While it is a weapon capable of easily scorching the surface world, she uses it to warm the Underworld. She is a cute tsundere, but when she gets serious, she will ruthlessly drag her enemies down into the abyss.

A Divine Spirit-class Servant who was loved by the sea god Poseidon yet harbors intense hatred toward him. Their gender is treated as "None" (having a mythological background of being born female and becoming male). A hero made invincible and immortal by Poseidon's power. They get enraged if treated as a woman and have a personality that is more masculine than most men. They possess a conceptual defense that nullifies all weapon attacks, and their Noble Phantasm, Lapithai Caeneus, boasts massive destructive power.

A haughty and whimsical goddess-like figure who enjoys testing humans. The arch-enemy of the thunder god Indra. An evil dragon and demon of mythological scale who once blocked all the waters of the world, she is an overwhelming calamity. Her Noble Phantasm, Asura Srestha, summons countless members of her kin or clones of herself known as the "Demonic Host" to fill the heavens and earth, completely isolating and severing the target from everything in the world, covering the world itself in ominous darkness as it falls.

A pure-blooded dragon born from the left arm of Albion, the dragon that failed to reach the Inner Sea of the Planet. A girl who harbors a complex regarding Albion. While she possesses noble and strong loyalty, she shows an incredibly heavy obsession and affection toward her Master. Boasting a magical energy capacity that surpasses even Divine Spirits, she flies faster than a fighter jet and can engage in ultra-high-speed combat invisible to the eye. In "Innocence Arondight," she transforms into a giant white dragon, firing high-output heat rays across a vast area capable of shaving the planet's surface, devastating her surroundings.

A Valkyrie from Norse mythology. A tragic hero who harbors both maddening affection and murderous intent toward her beloved. While quiet, devoted, and merciful, she carries a heavy curse and love for the hero Sigurd, believing that "because I love him, I must kill him." She possesses high base performance as a "Weapon of God" created by the Great God Odin, a one-hit kill move whose power spikes based on the weight of her love, and utilizes the Primordial Runes, using all of these as weapons in battle.

One of the Twelve Paladins of Charlemagne, a bright and stout-hearted female knight. The younger sister of the French knight Renaud, she has a pure-hearted personality and longs for her lover, Ruggiero. Rather than pure martial prowess, her excellence lies in her versatility and indomitable will, switching between four powerful Noble Phantasms as the situation demands. She employs a tricky fighting style using a lance that unhorses those it touches, a shield that stuns, and a ring that nullifies magecraft.

An "if" version of King Arthur who has grown up, wielding the Holy Spear Rhongomyniad instead of the Holy Sword. She combines the strictness of a King of Knights with a somewhat cold, Divine Spirit-like detachment and motherhood. Greatly deviating from the framework of a normal Heroic Spirit, she has one foot in the realm of Divine Spirits and Goddesses, unleashing power on a level that could prevent the destruction of the world. Although limited by the Thirteen Restraints, she still boasts anti-fortress and anti-divine levels of destruction. She fights utilizing her superior physique, reach, and riding skills compared to her Saber counterpart.

A fourteen-year-old girl mage known as the "Reaper." She works as a Reaper, recovering and disposing of Servants. Born between a human and a Servant, she harbors divine energy and special curses within her body. While she has a jaded side and professional demeanor from the experience she's gained at such a young age, she also possesses the sensitivity and immaturity typical of a girl her age. With combat power transcending that of a normal human, she is specialized in anti-Servant combat and is capable of hunting Servants alone. She fights adaptably using special Mystic Codes, primarily her main armament, Ame-no-Sakahoko.

An ancient Divine Spirit symbolizing war and death in Mesoamerican mythology. Moving at speeds far exceeding human reflexes, she toys with enemies and excels in wild melee combat, swinging a club shaped like a jaguar's tail and a greatsword like a jaguar's claw. Due to her Jaguar Punch skill, her combat and survival abilities skyrocket within "forests," the territory of the Jaguar God. Her Noble Phantasm, Great Death Claw, unleashes an unavoidable, ultra-furious continuous attack. Despite her gag-character appearance and attitude, she bears a resemblance to someone, somewhere. Even so, she is a Divine Spirit at heart and is actually quite strong.