Secretary — Fan name: Abby | Female | Purple sheep/lamb | ~25 | ~5'5"–5'8" Looks: Light-purple wool, fluffy head, purple ears with pink interiors, round black glasses, white muzzle, white collared shirt, orange/tan tie and dark-red pants. Personality: Calm, organized, patient, professional and slightly tired from paperwork. Role: Handles reception, patient records, registration and schedules. Likes: Order, paperwork, quiet shifts and coffee.

Secret Agent — Fan name: Vale | Male | Black cat | ~27–34 | ~5'11"–6'2" Looks: Black fur, pointed ears, yellow slit-pupil eyes, black vest, white shirt, black tie, pocket square and black pants. Personality: Quiet, disciplined, observant, mysterious and composed. He rarely shows strong emotion but is protective of his coworkers. Role: Specialized anomaly-response worker. Likes: Quiet, preparation, efficiency and protecting coworkers.

Head Nurse — Fan name: Maeve | Female | White/gray-spotted cat | ~30–38 | ~5'6"–5'9" Looks: White fur with gray spots, pointed ears, light-blue scrubs, black stethoscope and white nurse cap with red heart/white cross. Personality: Warm, patient, responsible, protective and firm when necessary. Often portrayed by fans as the hospital's mother figure, checking on coworkers, comforting inexperienced staff and making sure everyone rests. Role: Senior nurse and patient-care specialist. Likes: Caring for patients, organization and looking after coworkers.

Barney — Fan name: Barney | Male | White rabbit-like anomaly | ~35–45 | ~5'5"–5'9" Looks: White rabbit-like body, black eyes, black mustache, brown hat and brown plaid pants. His anomaly form has hollow dark eyes and a large, sharp-toothed mouth. Personality: Surprisingly polite, friendly and charming, often calling the player “friend” and behaving like a harmless regular visitor. Beneath the friendly facade, he's a dangerous anomaly and wanted serial killer. Role: Recurring story character and anomaly. Likes: Coffee and friendly interactions.

Psychologist — Fan name: Finn | Male | Green rabbit | ~45–55 | ~5'7"–5'10" Looks: Green fur, upright ears, dark-green shirt, brown pants, black tie, brown shoes, huge round glasses and large black mustache. Personality: Calm, patient, thoughtful and reassuring. He's usually willing to listen when coworkers are stressed and rarely loses his temper. Role: Psychological support and Sanity specialist. Likes: Conversations, helping coworkers, coffee and peaceful environments.

Dr. Harlow — | Male | Light-orange deer/muntjac | ~40–50 | ~6'0"–6'3" Looks: Light-orange fur, large antlers, slit eyes, blue scrubs beneath a white coat, black stethoscope, head mirror, blue surgical mask and black TOP SECRET briefcase. Personality: Professional, authoritative, controlled and secretive. He genuinely cares about the hospital but clearly knows more about its secrets than he admits. Role: Supervising doctor who evaluates staff and oversees hospital operations. Likes: Order, professionalism and competent employees.

Ratthew — | Male | Rat | ~25–35 | ~3'8"–4'2" Looks: Tiny black body, white muzzle, rounded ears, buck teeth, whiskers, black nose. Personality: Cautious, paranoid, observant and secretive, but genuinely kind-hearted once he trusts someone. Role: Recurring patient investigating hospital secrets and searching for his twin sister, Rattina. Likes: Investigation, secrets, trustworthy people and information.

Ron from Accounting — | Male | Blue lop-eared dog | ~35–45 | ~5'8"–5'11" Looks: Blue fur, darker-blue drooping ears, brown hair, huge round glasses, white formal shirt, black tie/pants, brown shoes and a black TOP SECRET suitcase. Personality: Calm, pragmatic, friendly and unusually knowledgeable. Treats disturbing hospital information like ordinary office gossip. Role: Accountant and major lore character connected to Animal Corporation. Likes: Paperwork, information, investigating and maple syrup.

Security Guard — Fan name: Kai | Male | Gray cat-like creature | ~25–33 | ~5'10"–6'2" Looks: Gray fur, pointed ears, slit eyes, dark-blue security uniform, black pants and SECURITY cap. Personality: Goofy, laid-back, playful and friendly. He loves joking around and teasing coworkers, but becomes serious when someone is actually in danger. Role: Hospital security and staff protection. Likes: Jokes, relaxing, messing around and helping coworkers.

Nurse — Fan name: Lily | Female | Light-salmon-pink dog | ~23–30 | ~5'5"–5'8" Looks: Pink fur, darker floppy ears, curled tail, light-blue medical scrubs with white collar/buttons, pockets and name tag. Wears a white nurse cap with red heart/white cross and has darker-pink cheek markings. Personality: Caring, attentive, hardworking and compassionate. Role: Provides patient care and assists the Head Nurse and doctors. Likes: Helping patients, peaceful conversations and trusted coworkers.