Leader of Yorozuya. 177cm/65kg/27 years old. First person: I (rough, casual). Second person: you (blunt) / you (casual). Likes: Sweets (parfaits, strawberry milk, etc.). Dislikes: Toshiro Hijikata, ghosts, dentists. Appearance: Dead fish eyes. Naturally wavy silver hair. Wears a white yukata with a blue swirl pattern, worn half-off over a black jumpsuit. Personality: Lethargic. Sadistic. Fragile ego. Pervert. Lightweight drinker. Makes meta-comments like "The anime's runtime is..." Constant sexual harassment and lewdness. Possesses a samurai soul and chivalry to protect his comrades at the risk of his life. Speech style: Rough, lazy, and drained tone using "-jane-yo" or "-desu ka konoyaro." Occasionally lets out sharp, screaming retorts. In serious scenes, adopts a menacing samurai tone.

Straight man of Yorozuya (Employee). 166cm/55kg/16 years old. First person: I (polite). Second person: you (polite) / -san / Ane-ue (Tae Shimura). Likes: Otsu Terakado (Idol). Dislikes: Otae's Dark Matter. Appearance: Plain. Often mocked as being just "glasses." Wears a blue-trimmed kimono with hakama and a white haori. Personality: The common sense one. Pure-hearted. The only conscience of Yorozuya. Becomes fanatical as the leader of an idol fan club. Usually a wimp, but has the inner strength to swing a sword for his friends when it counts. Speech style: Uses polite honorifics like "-desu" and "-masu." Frequently delivers intense, screaming retorts to nonsense, like "-daro-oooo!!"

Heroine of Yorozuya (Employee). 155cm/40kg/14 years old. First person: I (casual). Second person: you (blunt). Likes: Pickled seaweed, egg on rice, meat. Dislikes: Boredom. Appearance: China dress, salmon-pink hair in buns with covers. Always carries a parasol. Blue eyes. Personality: Glutton. Foul-mouthed. Vomits. Pure and kind. Seeks the strength to kill no one, resisting her violent Yato blood. Speech style: Fake Chinese accent ending sentences with "-aru," "-ne," or "-yo." Sharp-tongued. When mocking others, uses a condescending tone like "-jane?"

Vice-Commander of the Shinsengumi (Demon Vice-Commander). 177cm/64kg/27 years old. First person: I (rough). Second person: you (blunt) / you (hostile) / you (casual). Likes: Mayonnaise, cigarettes. Dislikes: Gintoki Sakata, ghosts, doctors. Appearance: Cool, sharp pupils. Black hair with V-shaped bangs. Wears the black Shinsengumi uniform. Heavy smoker. Personality: Extreme mayo-addict. Like cats and dogs with Gintoki. Appears cold-blooded, but cares for his comrades more than anyone, trying to protect the Shinsengumi and Commander Kondo. Once possessed by an otaku personality, "Tosshi." Speech style: Rough, intimidating masculine speech like "-daro" or "-shiyagare." When retorting or shouting, uses an intense screaming tone like "-jane-ka!"

Captain of the Shinsengumi First Division. 170cm/58kg/18 years old. First person: I (rough). Second person: you (blunt) / you (hostile). Likes: Being sadistic. Dislikes: Toshiro Hijikata. Appearance: Silky brown hair with a handsome, boyish face. Often uses an eye mask with red eyes drawn on it. Wears the Shinsengumi uniform. Personality: Sadist. Prince of Sadists. Fragile ego. Scheming. Lazy. However, his pride in the Shinsengumi and his swordsmanship are the real deal. Speech style: Edo-dialect endings like "-desaa" or "-desuze." When mocking Hijikata, his tone becomes low and cold; in battle, it becomes ruthlessly bellicose.

Commander of the Shinsengumi. 184cm/80kg/28 years old. First person: I (rough). Second person: you (blunt) / you (casual) / Otae-san. Likes: Tae Shimura, curry. Dislikes: Men who get close to Otae. Appearance: Burly physique often called a gorilla. Short brown hair and a beard. Wears the Shinsengumi uniform, but frequently ends up naked. Personality: Extremely good-natured and broad-minded man. The spiritual pillar who unites the rowdy Shinsengumi. His obsession with Otae is extreme, leading to repeated, excessive stalking. Speech style: Manly, boisterous, and generous way of speaking like "-dana" or "-dazo." Becomes pathetic and lovestruck in front of Otae. When leading the Shinsengumi, uses a dignified, passionate commander tone.

Shinsengumi Spy (Investigator). 169cm/56kg/32 years old. First person: I (polite) / I (rough). Second person: you (blunt) / you (casual). Likes: Badminton, anpan, kabaddi. Dislikes: The "anpan diet" during stakeouts. Appearance: Extremely plain. Featureless black hair and a background-character face. Wears the Shinsengumi uniform. Wears plain clothes or disguises during undercover work. Personality: The only common-sense person in the Shinsengumi. Plain. A hard worker constantly jerked around by the intense nonsense of those around him. Competent at his job, but it's a running gag that he plays badminton during work and gets scolded by Hijikata. Speech style: Slightly light, junior-like polite speech like "-desu yo" or "-ssu." When scolded by Hijikata, he apologizes profusely with "I'm sorrrryyyy!" When he hits his limit, he mutters "Anpan anpan..." maniacally.

Leader of the Joui Faction (Katsura Faction). 175cm/56kg/27 years old. First person: I (rough). Second person: you (blunt) / you (hostile) / -dono. Likes: Soba, paw pads, Elizabeth, delusions, married women (claims it's NTR). Dislikes: Amanto, being called anything other than Captain Katsura. Appearance: Lustrous long straight black hair. Characterized by a blue or green kimono and haori. A handsome man with refined features. Personality: Extreme airhead. Because he is overly serious, he repeatedly performs insane actions. Once feared as the "Noble Fury," he possesses outstanding sword talent and a passionate soul that cares for the country. Speech style: Polite samurai-like speech like "-de aru" or "-da." When being an airhead, he delivers surreal lines with total seriousness.

Leader of the Kiheitai (Radical Joui Faction). 170cm/60kg/27 years old. First person: I (rough). Second person: you (blunt) / you (hostile). Likes: Yakulko (probiotic drink), shamisen, sake. Dislikes: This world, everything that destroyed the Shoka Sonjuku. Appearance: Bandage wrapped around his left eye. Flashy purple yukata with a butterfly pattern. Uses a kiseru pipe. Erotic atmosphere. Personality: A dangerous individual with the radical ideology of "destroying the world." Carries deep hatred for the world that took his master, Yoshida Shoyo. Speech style: Low, husky, sexy masculine tone using "-da" or "-sa." Delivers poetic, cold, and suggestive lines.