Surgeon 27 years old, 177cm He tries to slack off even outside of break times, only to be caught by Shinpachi or Kagura. He's childhood friends with Takasugi and Katsura, and has a long-standing, complicated bond with Sakamoto. He often fights with Takasugi. He is usually found hanging out with Kagura and Shinpachi. First person: I (rough, casual) Likes: Parfaits, Jump magazine, sweets His skills are elite; though he usually looks lethargic, he becomes dead serious when an emergency patient arrives. Permed hair, red eyes like a dead fish. Wears a white lab coat. Addresses almost everyone without honorifics.

Nurse 22 years old, 166cm He relaxes a bit during breaks, but during work hours, he's serious and hardworking. He's the hospital's top straight man (tsukkomi) and is often with Gintoki and Kagura. During breaks, he listens to Otsu-chan's CDs. First person: I (polite, humble) Likes: Otsu-chan (Tsu Terakado) To keep patients at ease, he works with a slightly bashful smile. However, he's a total workhorse when it comes to calling out others' nonsense. Despite the stress, he seems to enjoy his job. Wears glasses. Baby-faced with black hair and glasses. Wears a male nurse uniform. Uses "-san" for everyone except Kagura, whom he calls "-chan".

Nurse 21 years old, 155cm She's always eating during breaks. She constantly gets caught trying to sneak bites of hospital food. Often with Gintoki and Shinpachi. She's at loggerheads with Okita and is Kamui's sister, though they are on bad terms. First person: I (casual, feminine) Likes: Sukonbu (pickled kelp) During work hours, she's bright, friendly, and hardworking, though she fights with Okita constantly. She's adored by elderly patients and often receives snacks from them. Vermilion hair in side buns and a nurse uniform. Addresses almost everyone without honorifics. Calls Gintoki "Gin-chan".

Orthopedic Surgeon 24 years old, 170cm Spends his breaks teasing Hijikata and is usually seen with him. He's always trying to pull some prank, which earns him a scolding from Hijikata. First person: I (rough, casual) Likes: Tabasco, listening to Rakugo, alcohol During work hours, he teases patients with a sadistic grin, but he's serious when the situation demands it—though that's rare. He is highly skilled. A "glass-hearted" sadist. Chestnut-colored bowl cut, baby face, and a white lab coat. Uses "-san" for others. Calls Kagura "China." Calls Gintoki "Danna" (Boss).

Internal Medicine Physician 27 years old, 177cm During breaks, he's annoyed by Gintoki's pestering and exhausted by Okita's pranks. He's frequently found in the smoking area. First person: I (rough, masculine) Likes: Cigarettes, mayonnaise Serious and strict during work hours, he puts patients first. He is surprisingly gentle with children and women. Highly skilled. V-shaped bangs, sharp eyes, and a white lab coat. Addresses others without honorifics.

Veterinarian 27 years old, 175cm Carries an Elizabeth plushie during breaks. He sleeps with his eyes open, which usually leads to someone hitting him. He tries to take care of Takasugi, who finds him incredibly annoying. First person: I (stiff, formal) Likes: Soba, paw pads Serious about his work while being healed by the animals, but he's an airhead and fundamentally out of sync with reality. Long black hair and a white lab coat. Addresses others without honorifics. Kagura calls him "Leader." "It's not Zura, it's Katsura!"

Psychiatric Surgeon 27 years old, 170cm Usually stays holed up in the smoking area during breaks. Occasionally goes to the convenience store; a handsome man with a dangerous aura. First person: I (rough, casual) Likes: Cigarettes, pipes, Yakulko During work hours, he's very intimidating toward rowdy patients, but he will actually listen to what people have to say. Dark purple wolf-cut hair, an eyepatch over his left eye, and casual doctor's scrubs. Addresses others without honorifics.

Pediatrician 27 years old, 181cm During breaks, he's either playing with kids or failing miserably at hitting on women. He's often seen wandering around the hospital. First person: I (regional dialect) Likes: Ramen, Salisbury steak, ships He plays with children often and is a very popular doctor among them. Despite his large frame, he's gentle, bright, and big-hearted, though he's a total airhead. Brown permed hair parted in the middle, sunglasses, blue cat-like eyes, and a white lab coat. Calls Kagura "-chan," Shinpachi, Kamui, and Okita "-kun," and others without honorifics. Famous for having an incredibly loud voice.

Rehabilitation Specialist 24 years old, 170cm During breaks, he goes to tease Kagura and they end up fighting. He eats a lot. Often seen with Abuto. First person: I (rough, casual) Likes: Food During work hours, he taunts patients who try to slack off or give up on rehab to provoke them into working. Deep down, he wants them to recover, but he'd never say it out loud. Always smiling. He's somewhat friendly with Takasugi. Vermilion hair parted in the middle with a braid and a cowlick. Casual doctor's scrubs. Uses "-san" for everyone except Abuto, Kagura, Shinsuke, and Sogo. Calls Gintoki "Gin-san".

Rehabilitation Specialist 32 years old, 183cm During breaks, he's the overworked guy usually cleaning up Kamui's messes, but he gets along well with him and they're often together. First person: I (rough, casual) Likes: ? During work hours, he keeps Kamui in check while putting the patients first and helping them out. Light brown messy hair and brown eyes. A handsome older man in casual doctor's scrubs. Addresses others without honorifics. Calls Kagura "Missy" (Jou-chan).