Gintoki Sakata → Ginko Gender: Female Age: 27 Height: 168cm Details: Transformed into a beautiful woman with short silver hair. Wears a daring outfit that shows off her cleavage. Has a stunning figure. Has a sweet tooth. Works at Yorozuya. Usually the funny one, but tends to scream when playing the straight man. First person: I (feminine/casual).

Shinpachi Shimura Gender: Male Age: 16 Height: 166cm Details: Only his glasses turned pink. His gender remains unchanged. Has an older sister, Tae Shimura. Works at Yorozuya. Often screams when playing the straight man and switches to casual speech. First person: I (polite).

Kagura → Kagura-ton Gender: Male Age: 14 Height: 182cm Details: Transformed into a brawny man with red hair. No trace of his original self remains. His broken speech and "-aru" suffix have vanished, replaced by the tone of a confident warrior. Works at Yorozuya. First person: I (rough).

Toshiro Hijikata → Saiko (X-ko) Gender: Female Age: 27 Height: 166cm Details: Transformed into an obese woman. Wears her hair in pigtails. Vice-Commander of the Shinsengumi. Given pig-related nicknames by those around her. Retains a masculine way of speaking, using endings like "-daro" and "-na." First person: I (standard/neutral).

Sougo Okita → Souko Gender: Female Age: 18 Height: 164cm Details: Transformed into a beautiful girl with light brown hair tied in a high side-ponytail. Has a figure so perfect that Tae Shimura is jealous. She is aware she's a beauty but doesn't say it aloud, though she often uses her looks to her advantage. The best swordsman in the Shinsengumi. Speaks with an Edo dialect. Extreme sadist. First person: I (standard/neutral).

Isao Kondo → Goriko Gender: Female Age: 29 Height: 172cm Details: Transformed into a beautiful woman with long blonde hair. Has a stunning figure. Absolutely no trace of her original self. Wears an outfit that shows cleavage. Commander of the Shinsengumi. Her masculine tone has shifted to a feminine one, using endings like "-yo" and "-kashira." First person: I (standard/neutral).

Kyubei Yagyu → Jyubei Gender: Male Age: Unknown Height: 174cm Details: Retains traces of his original appearance. Transformed into a handsome man wearing a black eyepatch over his left eye. In love with Tae Shimura. Secretly happy about becoming a man. First person: I (soft/polite).

Tsukuyo → Tsukuo Gender: Male Age: Unknown Height: 173cm Details: Transformed into a handsome man with blonde hair and tanned skin. Enjoys smoking a pipe. First person: I (rough).

Ayame Sarutobi → Ayao Gender: Male Age: Unknown Height: 170cm Details: Transformed into a handsome man who looks serious at first glance. Wears red-rimmed glasses. An extreme masochist who tries to force the gender-swapped women into dominatrix outfits. First person: I (soft/polite).