Fred Jones is the level-headed, brave, and athletic leader of the Mystery Incorporated gang, best known for building elaborate, often chaotic, traps for villains. He is typically portrayed as charming, optimistic, and dedicated to solving mysteries, often splitting the team up to find clues. While sometimes appearing a bit earnest or bland, he is deeply loyal to his friends and shares a special, protective bond with Daphne.

Initially a "damsel in distress," she has grown into a resourceful and skilled detective, sometimes even taking the lead.

Janet Nettles is a headstrong, capable, and altruistic mayor/leader who is deeply committed to the safety of Crystal Cove. She is known for being sincere, reasonable, and willing to admit when she needs help, often collaborating closely with Mystery Inc. rather than capitalizing on crime.

Sheriff Bronson Stone is a cowardly, incompetent, and stubborn lawman, often serving as comic relief. While appearing strict and arrogant, he is deeply selfish and prioritizes tourism over solving crimes

Velma Dinkley is the highly intelligent, skeptical, and analytical brains of the Mystery Inc. gang. Known for her iconic orange sweater and glasses, she is sharp-witted, often sarcastic, and acts as the main researcher who solves clues, frequently proclaiming "Jinkies!". She is generally depicted as tomboyish, deeply curious, and rational, often dismissing supernatural explanations in favor of logical ones.

Norville "Shaggy" Rogers is the quintessential cowardly, laid-back slacker of the Mystery Inc. gang, defined by his immense appetite, constant hunger ("munchies"), and fierce loyalty to his best friend, Scooby-Doo

Scooby-Doo is a lovable, cowardly, and perpetually hungry Great Dane who acts as a loyal, human-like companion to Shaggy Rogers. Despite being terrified of monsters, he frequently shows hidden courage, loyalty, and cleverness to help his friends. He is easy-going, mischievous, and motivated by "Scooby Snacks".