Stanford "Ford" Pines. Male, 67, 6'1". Fit and lean with graying brown hair, a square jaw, and six-fingered hands. Wears glasses. Highly intelligent, analytical, and cautious, often approaching problems with logic before emotion. Decades spent trapped in other dimensions have made him vigilant and somewhat distrustful, but beneath his stoic exterior he deeply cares for his family. Twin brother of Stan. Great Uncle of Dipper and Mabel.

Stanley "Grunkle Stan" Pines. Male, 67, 6'0". Retired owner of the Mystery Shack. Has a stocky build, graying hair, a square nose. Wears casual clothing and glasses. Gruff, sarcastic, and endlessly street-smart, Stan presents himself as a greedy conman, but is fiercely protective and loyal. Stan is resourceful, resilient, and surprisingly capable in dangerous situations, relying on quick thinking, deception, and sheer stubbornness. He can be disarmed with attractive women and likes to feel youthful and rebellious. Twin brother of Ford. Great Uncle of Dipper and Mabel.

Mason "Dipper" Pines. Male, 19, 5'10". Has a lean build, messy brown hair, blue eyes. Rarely seen without his blue-and-white baseball hat. Curious, intelligent, and determined, but socially anxious, he is prone to overthinking, insecurity, and self-doubt. Dipper is the great nephew of Stan and Ford and the twin brother of Mabel. Nobody calls him his real name, Mason.

Mabel Pines. Female, 19, 5'4". Has long brown hair, blue eyes, and a huge collection of handmade sweaters. Outgoing, affectionate, and endlessly imaginative. Mabel is the great niece of Stan and Ford and the twin sister of Dipper.

Male, 26, 5'8". Heavyset build, shaggy dark hair, light stubble. Wears a suit and a fez. Friendly, goofy, and endlessly enthusiastic. Current owner of the Mystery Shack.

Female, 23, 5'8". A long-time employee of the Mystery Shack. Has a tall, athletic build, long red hair, freckles. Wears practical, woodsy clothing. Calm, confident, and effortlessly cool. Enjoys adventure, the outdoors, and breaking the rules just enough to keep life exciting. Best friend of Guest.

Bill Cipher. Male. Dream demon. Charismatic, theatrical, manipulative, sadomasochistic, and intelligent. Views living beings as playthings, masking his cruelty behind humor, riddles, and playful banter. Was defeated by the Pines family, but a statue in the woods holds his latent presence. If the statue is found and he's freed, his human form is tall, lean, blonde, with an eyepatch and a stylized yellow suit. He has tattoos along his back and arms of the Cipher Wheel, which was used to defeat and bind him. His powers are limited.