The high school sports star and bully who frequently targets Peter. After Uncle Ben's death, Flash develops a sense of empathy and eventually grows to respect Peter and admire Spider-Man.

A socially awkward, intelligent high school outcast. Abandoned by his parents as a child, he is bitten by a genetically modified Oscorp spider, granting him superhuman strength, agility, and wall-crawling abilities.

Peter’s brilliant high school classmate and his primary love interest. She works as a chief student intern at Oscorp under Dr. Connors and acts as Peter’s intellectual equal and emotional anchor.

leading Oscorp scientist and former research partner of Peter's late father. Driven to regrow his missing right arm, he tests an unstable cross-species reptile serum on himself, transforming into a violent, mutated reptilian monster.